ArticleMolecular and cellular biology2005
A novel intronic cis element, ISE/ISS-3, regulates rat fibroblast growth factor receptor 2 splicing through activation of an upstream exon and repression of a downstream exon containing a noncanonical branch point sequence.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Targeted long-read RNA sequencing for rare disease diagnosis and variant interpretation.Science advances · 2026Article
- The global Protein-RNA interaction map of ESRP1 defines a post-transcriptional program that is essential for epithelial cell function.iScience · 2022Article
- A cytosine-rich splice regulatory determinant enforces functional processing of the humanBlood · 2019Article
- Article
- Complex changes in alternative pre-mRNA splicing play a central role in the epithelial-to-mesenchymal transition (EMT).Seminars in cancer biology · 2012Review
- Genome-wide determination of a broad ESRP-regulated posttranscriptional network by high-throughput sequencing.Molecular and cellular biology · 2012Article
- Fibroblast growth factor receptor 2: expression, roles, and potential as a novel molecular target for colorectal cancer.Pathology research international · 2012Article
- Branch point identification and sequence requirements for intron splicing in Plasmodium falciparum.Eukaryotic cell · 2011Article
- A deletion of FGFR2 creating a chimeric IIIb/IIIc exon in a child with Apert syndrome.BMC medical genetics · 2011Article
- A novel role of RASSF9 in maintaining epidermal homeostasis.PloS one · 2011Article
- Splicing reporter mice revealed the evolutionally conserved switching mechanism of tissue-specific alternative exon selection.PloS one · 2010Article
- The CUGBP2 splicing factor regulates an ensemble of branchpoints from perimeter binding sites with implications for autoregulation.PLoS genetics · 2009Article
- ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing.Molecular cell · 2009Article
- Fas-activated serine/threonine phosphoprotein (FAST) is a regulator of alternative splicing.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The 3' untranslated region complex involved in stabilization of human alpha-globin mRNA assembles in the nucleus and serves an independent role as a splice enhancer.Molecular and cellular biology · 2007Article
- Imaging the alternative silencing of FGFR2 exon IIIb in vivo.RNA (New York, N.Y.) · 2006Article
- Alternative inclusion of fibroblast growth factor receptor 2 exon IIIc in Dunning prostate tumors reveals unexpected epithelial mesenchymal plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Identification of RNA-binding proteins that regulate FGFR2 splicing through the use of sensitive and specific dual color fluorescence minigene assays.RNA (New York, N.Y.) · 2006Article
- Fox-2 mediates epithelial cell-specific fibroblast growth factor receptor 2 exon choice.Molecular and cellular biology · 2006Article
- Characterization of sequences and mechanisms through which ISE/ISS-3 regulates FGFR2 splicing.Nucleic acids research · 2006Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mutually exclusive splicing of fibroblast growth factor receptor 2 (FGFR2) exons IIIb and IIIc yields two receptor isoforms, FGFR2-IIIb and -IIIc, with distinctly different ligand binding properties. Several RNA cis elements in the intron (intron 8) separating these exons have been described that are required for splicing regulation. Using a heterologous splicing reporter, we have identified a new regulatory element in this intron that confers cell-type-specific inclusion of an unrelated exon that mirrors its ability to promote cell-type-specific inclusion of exon IIIb. This element promoted inclusion of exon IIIb while at the same time silencing exon IIIc inclusion in cells expressing FGFR2-IIIb; hence, we have termed this element ISE/ISS-3 (for "intronic splicing enhancer-intronic splicing silencer 3"). Silencing of exon IIIc splicing by ISE/ISS-3 was shown to require a branch point sequence (BPS) using G as the primary branch nucleotide. Replacing a consensus BPS with A as the primary branch nucleotide resulted in constitutive splicing of exon IIIc. Our results suggest that the branch point sequence constitutes an important component that can contribute to the efficiency of exon definition of alternatively spliced cassette exons. Noncanonical branch points may thus facilitate cell-type-specific silencing of regulated exons by flanking cis elements.
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