ArticleProceedings of the National Academy of Sciences of the United States of America2004
Responses of fibroblasts to anchorage of dorsal extracellular matrix receptors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
94 citing papers in PubMed, 267 citations in OpenAlex.
- The Floor-Ceiling-Chip, or 2 × 2D = Pseudo-3D-Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell-Adhesive Protein Micropatterns.Advanced healthcare materials · 2026Article
- Matrix viscoelasticity regulates dermal fibroblast activation in a three-dimensional fibrillar microenvironment.bioRxiv : the preprint server for biology · 2026Article
- A self-assembled protein β-helix as a self-contained biofunctional motif.Nature communications · 2025Article
- Prospects and challenges of ovarian cancer organoids in chemotherapy research (Review).Oncology letters · 2025Review
- Electroactive 4D Porous Scaffold Based on Conducting Polymer as a Responsive and DynamicACS applied materials & interfaces · 2024Article
- Advanced Mechanical Testing Technologies at the Cellular Level: The Mechanisms and Application in Tissue Engineering.Polymers · 2023Review
- Aspirin Affects MDA-MB-231 Vesicle Production and Their Capacity to Induce Fibroblasts towards a Pro-Invasive State.International journal of molecular sciences · 2023Article
- Three-Dimensional Evaluation of the Cytotoxicity and Antibacterial Properties of Alpha Lipoic Acid-Capped Silver Nanoparticle Constructs for Oral Applications.Nanomaterials (Basel, Switzerland) · 2023Article
- Smart biomaterial platforms: Controlling and being controlled by cells.Biomaterials · 2022Review
- Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture.ACS biomaterials science & engineering · 2021Review
- Prostaglandin F2α Agonists Negatively Modulate the Size of 3D Organoids from Primary Human Orbital Fibroblasts.Investigative ophthalmology & visual science · 2020Article
- Evaluation of Dental Pulp Stem Cell Heterogeneity and Behaviour in 3D Type I Collagen Gels.BioMed research international · 2020Article
- Adaptable boronate ester hydrogels with tunable viscoelastic spectra to probe timescale dependent mechanotransduction.Biomaterials · 2019Article
- Bridging 2D and 3D culture: probing impact of extracellular environment on fibroblast activation in layered hydrogels.AIChE journal. American Institute of Chemical Engineers · 2019Article
- Coordinated increase of nuclear tension and lamin-A with matrix stiffness outcompetes lamin-B receptor that favors soft tissue phenotypes.Molecular biology of the cell · 2017Article
- Probing Cell Adhesion Profiles with a Microscale Adhesive Choice Assay.Biophysical journal · 2017Article
- Article
- Confined Sandwichlike Microenvironments Tune Myogenic Differentiation.ACS biomaterials science & engineering · 2017Article
- MCAK-mediated regulation of endothelial cell microtubule dynamics is mechanosensitive to myosin-II contractility.Molecular biology of the cell · 2017Article
- A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitro.International journal of molecular medicine · 2017Article
34 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Fibroblasts in 2D cultures differ dramatically in behavior from those in the 3D environment of a multicellular organism. However, the basis of this disparity is unknown. A key difference is the spatial arrangement of anchored extracellular matrix (ECM) receptors to the ventral surface in 2D cultures and throughout the entire surface in 3D cultures. Therefore, we asked whether changing the topography of ECM receptor anchorage alone could invoke a morphological response. By using polyacrylamide-based substrates to present anchored fibronectin or collagen on dorsal cell surfaces, we found that well spread fibroblasts in 2D cultures quickly changed into a bipolar or stellate morphology similar to fibroblasts in vivo. Cells in this environment lacked lamellipodia and large actin bundles and formed small focal adhesions only near focused sites of protrusion. These responses depend on substrate rigidity, calcium ion, and, likely, the calcium-dependent protease calpain. We suggest that fibroblasts respond to both spatial distribution and mechanical input of anchored ECM receptors. Changes in cell shape may in turn affect diverse cellular activities, including gene expression, growth, and differentiation, as shown in numerous previous studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.