ArticleThe Journal of cell biology1992
A synthetic peptide derived from the carboxy terminus of the laminin A chain represents a binding site for the alpha 3 beta 1 integrin.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 135 citations in OpenAlex.
- Extracellular vesicle-based delivery to airway basal cells for durable gene therapy in cystic fibrosis.Frontiers in bioengineering and biotechnology · 2026Review
- Dystroglycan-HSPG interactions provide synaptic plasticity and specificity.Glycobiology · 2024Review
- Regulation of human nucleus pulposus cells by peptide-coupled substrates.Acta biomaterialia · 2017Article
- Glucose autoxidation induces functional damage to proteins via modification of critical arginine residues.Biochemistry · 2011Article
- A biologically active sequence of the laminin alpha2 large globular 1 domain promotes cell adhesion through syndecan-1 by inducing phosphorylation and membrane localization of protein kinase Cdelta.The Journal of biological chemistry · 2009Article
- Discovery of targeting ligands for breast cancer cells using the one-bead one-compound combinatorial method.Journal of medicinal chemistry · 2009Article
- Integrin alpha3beta1 is an alternative cellular receptor for adenovirus serotype 5.Journal of virology · 2003Article
- Functional peptide sequences derived from extracellular matrix glycoproteins and their receptors: strategies to improve neuronal regeneration.Molecular neurobiology · 2003Review
- Cell contact-dependent activation of alpha3beta1 integrin modulates endothelial cell responses to thrombospondin-1.Molecular biology of the cell · 2000Article
- Division of labor among the alpha6beta4 integrin, beta1 integrins, and an E3 laminin receptor to signal morphogenesis and beta-casein expression in mammary epithelial cells.Molecular biology of the cell · 1999Article
- Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow.British journal of cancer · 1999Article
- Neuronal receptors mediating responses to antibodyactivated laminin-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- The role of laminins in basement membrane function.Journal of anatomy · 1998Review
- alphavbeta3 integrin mediates the cell-adhesive capacity and biological activity of basic fibroblast growth factor (FGF-2) in cultured endothelial cells.Molecular biology of the cell · 1997Article
- Identification of a novel contactin-associated transmembrane receptor with multiple domains implicated in protein-protein interactions.The EMBO journal · 1997Article
- Integrin alpha 3 beta 1 participates in the phagocytosis of extracellular matrix molecules by human breast cancer cells.Molecular biology of the cell · 1996Article
- The use of a combinatorial library method to isolate human tumor cell adhesion peptides.Molecular diversity · 1996Article
- Extracellular matrix remodeling and the regulation of epithelial-stromal interactions during differentiation and involution.Kidney international. Supplement · 1996Review
- Role of laminin and integrin interactions in growth cone guidance.Molecular neurobiology · 1996Review
- CD44/chondroitin sulfate proteoglycan and alpha 2 beta 1 integrin mediate human melanoma cell migration on type IV collagen and invasion of basement membranes.Molecular biology of the cell · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The purpose of this study was to identify the binding site(s) within laminin for the alpha 3 beta 1 integrin receptor. It has been previously shown, using proteolytic fragments and anti-laminin antibodies, that the region in laminin for alpha 3 beta 1 integrin binding is localized to the carboxy-terminal region at the end of the long arm (Gehlsen, K. R., E. Engvall, K. Dickerson, W. S. Argraves, and E. Ruoslahti. 1989. J. Biol. Chem. 264:19034-19038; Tomaselli, K. J., D. E. Hall, L. T. Reichardt, L. A. Flier, K. R. Gehlsen, D. C. Turner, and S. Carbonetto. 1990. Neuron. 5:651-662). Using synthetic peptides, we have identified an amino acid sequence within the carboxy-terminal region of the laminin A chain that is recognized by the alpha 3 beta 1 integrin. The amino acid sequence represented by the synthetic peptide GD-6 (KQNCLSSRASFRGCVRNLRLSR residues numbered 3011 to 3032) of the globular domain of the murine A chain supports cell attachment and inhibits cell adhesion to laminin-coated surfaces. By affinity chromatography, peptide GD-6-Sepharose specifically bound solubilized alpha 3 beta 1 from extracts of surface-iodinated cells in a cation-dependent manner, while it did not bind other integrins. In addition, exogenous peptide GD-6 specifically eluted bound alpha 3 beta 1 from laminin-Sepharose columns but did not elute the alpha 3 beta 1 integrin from a fibronectin-Sepharose column. Using integrin subunit-specific monoclonal antibodies, only those antibodies against the alpha 3 and beta 1 subunits inhibited cell adhesion to peptide GD-6-coated surfaces. Finally, a polyclonal antibody made against peptide GD-6 reacted specifically with both murine and human laminin and significantly inhibited cell adhesion to laminin-coated surfaces but not those coated with other matrix proteins. These results identify the laminin A chain amino acid sequence of peptide GD-6 as representing a binding site in laminin for the alpha 3 beta 1 integrin.
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