ArticleThe Journal of cell biology1992
Hypertrophic chondrocytes undergo further differentiation in culture.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 194 citations in OpenAlex.
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- Deficiencies in corin and atrial natriuretic peptide-mediated signaling impair endochondral ossification in bone development.Communications biology · 2024Article
- A bioactive composite scaffold enhances osteochondral repair by using thermosensitive chitosan hydrogel and endothelial lineage cell-derived chondrogenic cell.Materials today. Bio · 2024Article
- Independent mesenchymal progenitor pools respectively produce and maintain osteogenic and chondrogenic cells in zebrafish.Development, growth & differentiation · 2024Article
- Do Media Extracellular Vesicles and Extracellular Vesicles Bound to the Extracellular Matrix Represent Distinct Types of Vesicles?Biomolecules · 2023Review
- A Bioglass-Poly(lactic-co-glycolic Acid) Scaffold@Fibrin Hydrogel Construct to Support Endochondral Bone Formation.Advanced healthcare materials · 2023Article
- Transit Amplifying Cells (TACs): a still not fully understood cell population.Frontiers in bioengineering and biotechnology · 2023Review
- Article
- Chondrogenesis Defines Future Skeletal Patterns Via Cell Transdifferentiation from Chondrocytes to Bone Cells.Current osteoporosis reports · 2020Review
- Biochemical and histopathologic assessment of effects of acitretin on epiphyseal growth plate in rats.Postepy dermatologii i alergologii · 2020Article
- New morphological evidence of the 'fate' of growth plate hypertrophic chondrocytes in the general context of endochondral ossification.Journal of anatomy · 2020Article
- Osteochondrogenesis derived from synovial fibroblasts in inflammatory arthritis model.Inflammation and regeneration · 2020Review
- A Second Career for Chondrocytes-Transformation into Osteoblasts.Current osteoporosis reports · 2019Review
- Comparison between curcumin and all-trans retinoic acid in the osteogenic differentiation of mouse bone marrow mesenchymal stem cells.Experimental and therapeutic medicine · 2019Article
- Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models.Biochimica et biophysica acta. General subjects · 2018Review
- Non-sutural basicranium-derived cells undergo a unique mineralization pathway via a cartilage intermediatePeerJ · 2018Article
- The Chondro-Osseous Continuum: Is It Possible to Unlock the Potential Assigned Within?Frontiers in bioengineering and biotechnology · 2018Review
- Roles of Chondrocytes in Endochondral Bone Formation and Fracture Repair.Journal of dental research · 2017Review
- Deficiency and Also Transgenic Overexpression of Timp-3 Both Lead to Compromised Bone Mass and Architecture In Vivo.PloS one · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conditions have been defined for promoting growth and differentiation of hypertrophic chondrocytes obtained in culture starting from chick embryo tibiae. Hypertrophic chondrocytes, grown in suspension culture as described (Castagnola P., G. Moro, F. Descalzi Cancedda, and R. Cancedda. 1986. J. Cell Biol. 102:2310-2317), when they reached the stage of single cells, were transferred to substrate-dependent culture conditions in the presence of ascorbic acid. Cells showed a change in morphology, became more elongated and flattened, expressed alkaline phosphatase, and eventually mineralized. Type II and X collagen synthesis was halted and replaced by type I collagen synthesis. In addition the cells started to produce and to secrete in large amount a protein with an apparent molecular mass of 82 KD in reducing conditions and 63 KD in unreducing conditions. This protein is soluble in acidic solutions, does not contain collagenous domains, and is glycosylated. The Ch21 protein, a marker of hypertrophic chondrocytes and bone cells, was synthesized throughout the culture. We have defined this additional differentiation stage as an osteoblast-like stage. Calcium deposition in the extracellular matrix occurred regardless of the addition of beta glycerophosphate to the culture medium. Comparable results were obtained both when the cells were plated at low density and when they were already at confluence and maintained in culture without passaging up to 50 d. When retinoic acid was added to the hypertrophic chondrocyte culture between day 1 and day 5 the maturation of the cells to the osteoblast-like stage was highly accelerated. The switch in the collagen secretion was already observed after 2 d and the production of the 63-kD protein after 3 d. Mineralization was observed after 15-20 d.
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