ArticleGenes & development2005
UBF-binding site arrays form pseudo-NORs and sequester the RNA polymerase I transcription machinery.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 176 citations in OpenAlex.
- Nucleolar Dynamics During Oogenesis.bioRxiv : the preprint server for biology · 2026Article
- Chromosome-specific epigenetic control and transmission of ribosomal DNA arrays in Hominidae genomes.Cell genomics · 2025Article
- The Nucleolus: A Central Hub for Ribosome Biogenesis and Cellular Regulatory Signals.International journal of molecular sciences · 2025Review
- Emergent microenvironments of nucleoli.Nucleus (Austin, Tex.) · 2024Review
- Article
- Epigenetic control and inheritance of rDNA arrays.bioRxiv : the preprint server for biology · 2024Article
- Article
- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Review
- NORs on human acrocentric chromosome p-arms are active by default and can associate with nucleoli independently of rDNA.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Molecular Cytogenetic Analysis in Freshwater Prawns of the GenusInternational journal of molecular sciences · 2020Article
- Human NORs, comprising rDNA arrays and functionally conserved distal elements, are located within dynamic chromosomal regions.Genes & development · 2019Article
- Ribosomal DNA and the Nucleolus as Keystones of Nuclear Architecture, Organization, and Function.Trends in genetics : TIG · 2019Review
- Superresolution microscopy reveals linkages between ribosomal DNA on heterologous chromosomes.The Journal of cell biology · 2019Article
- Review
- The role of transcription in shaping the spatial organization of the genome.Nature reviews. Molecular cell biology · 2019Review
- The chromatin landscape of the ribosomal RNA genes in mouse and human.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Review
- Compositional reorganization of the nucleolus in budding yeast mitosis.Molecular biology of the cell · 2019Article
- Changes in long-range rDNA-genomic interactions associate with altered RNA polymerase II gene programs during malignant transformation.Communications biology · 2019Article
- Use of the iNo score to discriminate normal from altered nucleolar morphology, with applications in basic cell biology and potential in human disease diagnostics.Nature protocols · 2018Article
39 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Human ribosomal genes (rDNA) are located in nucleolar organizer regions (NORs) on the short arms of acrocentric chromosomes. Metaphase NORs that were transcriptionally active in the previous cell cycle appear as prominent chromosomal features termed secondary constrictions that are achromatic in chromosome banding and positive in silver staining. The architectural RNA polymerase I (pol I) transcription factor UBF binds extensively across rDNA throughout the cell cycle. To determine if UBF binding underpins NOR structure, we integrated large arrays of heterologous UBF-binding sequences at ectopic sites on human chromosomes. These arrays efficiently recruit UBF even to sites outside the nucleolus and, during metaphase, form novel silver stainable secondary constrictions, termed pseudo-NORs, morphologically similar to NORs. We demonstrate for the first time that in addition to UBF the other components of the pol I machinery are found associated with sequences across the entire human rDNA repeat. Remarkably, a significant fraction of these same pol I factors are sequestered by pseudo-NORs independent of both transcription and nucleoli. Because of the heterologous nature of the sequence employed, we infer that sequestration is mediated primarily by protein-protein interactions with UBF. These results suggest that extensive binding of UBF is responsible for formation and maintenance of the secondary constriction at active NORs. Furthermore, we propose that UBF mediates recruitment of the pol I machinery to nucleoli independently of promoter elements.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.