ArticleJournal of virology2005
La Crosse virus nonstructural protein NSs counteracts the effects of short interfering RNA.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 102 citations in OpenAlex.
- Virus meets RNAi. Symposium on antiviral applications of RNA interference.EMBO reports · 2008Trial
- Article
- Development of an RT-RPA assay for La Crosse virus detection provides insights into age-dependent neuroinvasion in mice.Virology journal · 2025Article
- Suppression of Interferon Response and Antiviral Strategies of Bunyaviruses.Tropical medicine and infectious disease · 2024Review
- Mammalian viral suppressors of RNA interference.Trends in biochemical sciences · 2022Review
- Antiviral RNA interference in disease vector (Asian longhorned) ticks.PLoS pathogens · 2021Article
- Slicing and dicing viruses: antiviral RNA interference in mammals.The EMBO journal · 2019Review
- Arboviruses and the Challenge to Establish Systemic and Persistent Infections in Competent Mosquito Vectors: The Interaction With the RNAi Mechanism.Frontiers in physiology · 2019Review
- A review on current status of antiviral siRNA.Reviews in medical virology · 2018Review
- Antiviral Immunity and Virus-Mediated Antagonism in Disease Vector Mosquitoes.Trends in microbiology · 2018Review
- Molecular mechanisms of Dicer: endonuclease and enzymatic activity.The Biochemical journal · 2017Review
- Virus-derived DNA drives mosquito vector tolerance to arboviral infection.Nature communications · 2016Article
- Review
- Analysis of Acquisition and Titer of Maize Mosaic Rhabdovirus in Its Vector, Peregrinus maidis (Hemiptera: Delphacidae).Journal of insect science (Online) · 2016Article
- Oropouche virus infection and pathogenesis are restricted by MAVS, IRF-3, IRF-7, and type I interferon signaling pathways in nonmyeloid cells.Journal of virology · 2015Article
- Review
- The NSs protein of tomato spotted wilt virus is required for persistent infection and transmission by Frankliniella occidentalis.Journal of virology · 2014Article
- Small interfering RNA inhibition of Andes virus replication.PloS one · 2014Article
- The role of innate immunity in conditioning mosquito susceptibility to West Nile virus.Viruses · 2013Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Through a process known as RNA interference (RNAi), double-stranded short interfering RNAs (siRNAs) silence gene expression in a sequence-specific manner. Recently, several viral proteins, including the nonstructural protein NSs of tomato spotted wilt virus (a plant-infecting bunyavirus), the interferon antagonist protein NS1 of influenza virus, and the E3L protein of vaccinia virus, have been shown to function as suppressors of RNAi, presumably as a counterdefense against cellular mechanisms that decrease viral production. La Crosse virus (LACV), a member of the California serogroup of orthobunyaviruses, has a trisegmented negative-stranded genome comprised of large (L), medium (M), and small (S) segments. To develop a strategy for segment-specific inhibition of transcription, we designed 13 synthetic siRNAs targeting specific RNA segments of the LACV genome that decreased LACV replication and antigen expression in mammalian (293T) and insect (C6/36) cells. Furthermore, NSs, a LACV nonstructural protein, markedly inhibited RNAi directed both against an LACV M segment construct and against a host gene (glyeraldehyde-3-phosphate dehydrogenase), suggesting a possible role for this viral protein in the suppression of RNA silencing. Segment-specific siRNAs will be useful as a tool to analyze LACV transcription and replication and to obtain recombinant viruses. Additionally, NSs will help us to identify molecular pathways involved in RNAi and further define its role in the innate immune system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.