ArticleThe Journal of general physiology2005
Perturbation analysis of the voltage-sensitive conformational changes of the Na+/glucose cotransporter.
Article in The Journal of general physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Different fluorescent labels report distinct components of spHCN channel voltage sensor movement.The Journal of general physiology · 2024Article
- Functional characterization of SGLT1 using SSM-based electrophysiology: Kinetics of sugar binding and translocation.Frontiers in physiology · 2023Article
- Functional characterization of a highly specific L-arabinose transporter from Trichoderma reesei.Microbial cell factories · 2021Article
- Active site voltage clamp fluorometry of the sodium glucose cotransporter hSGLT1.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Epithelial transport inThe Journal of general physiology · 2017Review
- The sodium/multivitamin transporter: a multipotent system with therapeutic implications.Vitamins and hormones · 2015Review
- Metadynamics simulations reveal a Na+ independent exiting path of galactose for the inward-facing conformation of vSGLT.PLoS computational biology · 2014Article
- Transepithelial glucose transport and Na+/K+ homeostasis in enterocytes: an integrative model.American journal of physiology. Cell physiology · 2014Article
- Forces and dynamics of glucose and inhibitor binding to sodium glucose co-transporter SGLT1 studied by single molecule force spectroscopy.The Journal of biological chemistry · 2014Article
- Correlating charge movements with local conformational changes of a Na(+)-coupled cotransporter.Biophysical journal · 2014Article
- The SLC34 family of sodium-dependent phosphate transporters.Pflugers Archiv : European journal of physiology · 2014Review
- Functional identification and characterization of sodium binding sites in Na symporters.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Simulated annealing reveals the kinetic activity of SGLT1, a member of the LeuT structural family.The Journal of general physiology · 2012Article
- A gate-free pathway for substrate release from the inward-facing state of the Na⁺-galactose transporter.Biochimica et biophysica acta · 2012Article
- Determination of the Na(+)/glucose cotransporter (SGLT1) turnover rate using the ion-trap technique.Biophysical journal · 2011Article
- Sucrose- and H-dependent charge movements associated with the gating of sucrose transporter ZmSUT1.PloS one · 2010Article
- Substrate-dependent proton antiport in neurotransmitter:sodium symporters.Nature chemical biology · 2010Article
- Prestin's anion transport and voltage-sensing capabilities are independent.Biophysical journal · 2009Article
- Effects on conformational states of the rabbit sodium/glucose cotransporter through modulation of polarity and charge at glutamine 457.Biophysical journal · 2009Article
- Transmembrane IV of the high-affinity sodium-glucose cotransporter participates in sugar binding.American journal of physiology. Cell physiology · 2008Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Conformational changes of the human Na(+)/glucose cotransporter (hSGLT1) were studied using voltage-jump methods. The cotransporter was expressed in Xenopus laevis oocytes, and SGLT1 charge movements were measured in the micro- to millisecond time scale using the cut-open oocyte preparation and in the millisecond to second time scale using the two-electrode voltage clamp method. Simultaneous charge and fluorescence changes were studied using tetramethylrhodamine-6-maleimide-labeled hSGLT1 Q457C. In 100 mM external [Na(+)], depolarizing voltage steps evoked a charge movement that rose initially to a peak (with time constant tau = 0.17 ms) before decaying to steady state with two time constants (tau = 2-30 and 25-150 ms). The time to peak (0.9 ms) decreased with [Na(+)], and was not observed in 0 mM [Na(+)]. In absence of Na(+), charge movement decayed monotonically to steady state with three time constants (0.2, 2, and 150 ms). Charge movement was accompanied by fluorescence changes with similar time courses, indicating that global conformational changes monitored by charge movement are reflected by local environmental changes at or near Q457C. Our results indicate that the major voltage-dependent step of the Na(+)/glucose transport cycle is the return of the empty carrier from inward to outward facing conformations. Finally, we observed subtle differences between time constants for charge movement and for optical changes, suggesting that optical recordings can be used to monitor local conformational changes that underlie the global conformational changes of cotransporters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.