ArticleBiophysical journal2005
Skeletal muscle NAD(P)H two-photon fluorescence microscopy in vivo: topology and optical inner filters.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 78 citations in OpenAlex.
- Mitochondrial Impairment in Unloaded Postural Muscle: Mechanisms Driving Loss of Muscle Function and Mass.Antioxidants (Basel, Switzerland) · 2026Review
- Multiphoton microscopy and tissue clearing for 3D characterization of the vasculature and fibrosis remodeling in rat dystrophic skeletal muscle.Scientific reports · 2025Article
- Impact of capillary and sarcolemmal proximity on mitochondrial structure and energetic function in skeletal muscle.The Journal of physiology · 2024Article
- Metabolic design in a mammalian model of extreme metabolism, the North American least shrew (Cryptotis parva).The Journal of physiology · 2022Article
- Energy metabolism design of the striated muscle cell.Physiological reviews · 2021Review
- Label-Free Multiphoton Microscopy: Much More Than Fancy Images.International journal of molecular sciences · 2021Review
- Subcellular Specialization of Mitochondrial Form and Function in Skeletal Muscle Cells.Frontiers in cell and developmental biology · 2021Review
- Association BetweenFrontiers in genetics · 2021Review
- How hot are single cells?The Journal of general physiology · 2020Article
- Visualizing Mitochondrial Form and Function within the Cell.Trends in molecular medicine · 2020Review
- Role of p110a subunit of PI3-kinase in skeletal muscle mitochondrial homeostasis and metabolism.Nature communications · 2019Article
- Mitochondrial matrix pH as a decisive factor in neurometabolic imaging.Neurophotonics · 2017Article
- Article
- Intravital multiphoton imaging of mouse tibialis anterior muscle.Intravital · 2016Article
- Article
- Use of independent component analysis to improve signal-to-noise ratio in multi-probe fluorescence microscopy.Journal of microscopy · 2014Article
- Motion compensation for in vivo subcellular optical microscopy.Journal of microscopy · 2014Review
- NADH changes during hypoxia, ischemia, and increased work differ between isolated heart preparations.American journal of physiology. Heart and circulatory physiology · 2014Article
- In vivo microscopy reveals extensive embedding of capillaries within the sarcolemma of skeletal muscle fibers.Microcirculation (New York, N.Y. : 1994) · 2014Article
- Compact non-contact total emission detection for in vivo multiphoton excitation microscopy.Journal of microscopy · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Two-photon excitation fluorescence microscopy (TPEFM) permits the investigation of the topology of intercellular events within living animals. TPEFM was used to monitor the distribution of mitochondrial reduced nicotinamide adenine dinucleotide (NAD(P)H) in murine skeletal muscle in vivo. NAD(P)H fluorescence emission was monitored ( approximately 460 nm) using 710-720 nm excitation. High-resolution TPEFM images were collected up to a depth of 150 microm from the surface of the tibialis anterior muscle. The NAD(P)H fluorescence images revealed subcellular structures consistent with subsarcolemmal, perivascular, intersarcomeric, and paranuclear mitochondria. In vivo fiber typing between IIB and IIA/D fibers was possible using the distribution and content of mitochondria from the NAD(P)H fluorescence signal. The intersarcomeric mitochondria concentrated at the Z-line in the IIB fiber types resulting in a periodic pattern with a spacing of one sarcomere (2.34 +/- 0.17 microm). The primary inner filter effects were nearly equivalent to water, however, the secondary inner filter effects were highly significant and dynamically affected the observed emission frequency and amplitude of the NAD(P)H fluorescence signal. These data demonstrate the feasibility, and highlight the complexity, of using NAD(P)H TPEFM in skeletal muscle to characterize the topology and metabolic function of mitochondria within the living mouse.
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