ArticleBiophysical journal2005
A two-photon FRAP analysis of the cytoskeleton dynamics in the microvilli of intestinal cells.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 56 citations in OpenAlex.
- High-resolution dynamic mapping of the C. elegans intestinal brush border.Development (Cambridge, England) · 2021Article
- Bioengineering tools for probing intracellular events in T lymphocytes.WIREs mechanisms of disease · 2021Review
- Profilin-Mediated Actin Allocation Regulates the Growth of Epithelial Microvilli.Current biology : CB · 2019Article
- Isochronal superpositioning in the equilibrium regime of superpressed propylene carbonate to ∼ 1.8 GPa: A study by diffusivity measurement of the fluorescent probe Coumarin 1.The European physical journal. E, Soft matter · 2019Article
- Numerical Simulation and FRAP Experiments Show That the Plasma Membrane Binding Protein PH-EFA6 Does Not Exhibit Anomalous Subdiffusion in Cells.Biomolecules · 2018Article
- Characterization of Cell Boundary and Confocal Effects Improves Quantitative FRAP Analysis.Biophysical journal · 2018Article
- The NHERF2 sequence adjacent and upstream of the ERM-binding domain affects NHERF2-ezrin binding and dexamethasone stimulated NHE3 activity.The Biochemical journal · 2015Article
- A repeat unit of Vibrio diarrheal T3S effector subverts cytoskeletal actin homeostasis via binding to interstrand region of actin filaments.Scientific reports · 2015Article
- Single- and two-photon fluorescence recovery after photobleaching.Cold Spring Harbor protocols · 2015Article
- Article
- Dynamics of ezrin and EBP50 in regulating microvilli on the apical aspect of epithelial cells.Biochemical Society transactions · 2014Article
- NHERF2 protein mobility rate is determined by a unique C-terminal domain that is also necessary for its regulation of NHE3 protein in OK cells.The Journal of biological chemistry · 2013Article
- Enterocyte loss of polarity and gut wound healing rely upon the F-actin-severing function of villin.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- PDZ interactions regulate rapid turnover of the scaffolding protein EBP50 in microvilli.The Journal of cell biology · 2012Article
- Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Multiphoton fluorescence recovery after photobleaching in bounded systems.Physical review. E, Statistical, nonlinear, and soft matter physics · 2011Article
- Diffusion measurements inside biofilms by image-based fluorescence recovery after photobleaching (FRAP) analysis with a commercial confocal laser scanning microscope.Applied and environmental microbiology · 2010Article
- High mobility of bicoid captured by fluorescence correlation spectroscopy: implication for the rapid establishment of its gradient.Biophysical journal · 2010Article
- A protein diffusion model of the sealing effect.Chemical engineering science · 2009Article
- Spatial distribution and mobility of the Ran GTPase in live interphase cells.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular structure of the brush-border of enterocytes has been investigated since the 1980s, but the dynamics of this highly specialized subcellular domain have been difficult to study due to its small size. To perform a detailed analysis of the dynamics of cytoskeleton proteins in this domain, we developed two-photon fluorescence recovery after photobleaching and a theoretical framework for data analysis. With this method, fast dynamics of proteins in the microvilli of the brush border of epithelial intestinal cells can be measured on the millisecond timescale in volumes smaller than 1 microm3. Two major proteins of the cytoskeleton of the microvilli, actin and myosin 1a (Myo1a; formerly named brush border myosin I), are mobile in the brush-border of Caco-2 cells, an enterocyte-like cellular model. However, the mobility of actin is very different from that of Myo1a and they appear to be unrelated (diffusion coefficient of 15 microm2 s(-1) with a mobile fraction of 60% for actin, and 4 microm2 s(-1) with a mobile fraction of 90% for Myo1a). Furthermore, we show for the first time, in vivo, that the dynamics of Myo1a in microvilli reflect its motor activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.