ArticleBiophysical journal2005
Electro-deformation and poration of giant vesicles viewed with high temporal resolution.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 265 citations in OpenAlex.
- Electric-field manipulation of giant vesicles: new frontiers in membrane electromechanics.European biophysics journal : EBJ · 2026Review
- [Coupling characteristics of cellular electroporation and electrodeformation based on dynamic Young's modulus: influence of membrane thickness].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2026Article
- Physical genomics: Why gene regulation is tug of war between polymer physics and biochemistry.Current opinion in structural biology · 2026Review
- Pulsed Electric Fields for Emerging Single-Cell Bioprocessing in Food Applications: Electropermeabilization Mechanisms and Design Principles.Comprehensive reviews in food science and food safety · 2026Review
- Electroporation in lipid vesicles by varying PEG-grafted lipids in their membranes: an experimental and simulation study.RSC advances · 2025Article
- Instability of a fluctuating biomimetic membrane driven by an applied uniform dc electric field.Physical review. E · 2025Article
- Electrodeformation of DMPC vesicle membranes near the main phase transition.Biophysical journal · 2025Article
- Diffuse-charge dynamics across a capacitive interface in a dc electric field.Physical review. E · 2025Article
- Synthesis Method and High Salt Concentration Can Affect Electrodeformation of GUVs under Strong Pulsed DC Fields.ACS omega · 2025Article
- Effects of Normal and Lateral Electric Fields on Membrane Mechanical Properties.The journal of physical chemistry. B · 2024Article
- Viscoelastic phenotyping of red blood cells.Biophysical journal · 2024Article
- Bilayer Charge Asymmetry and Oil Residues Destabilize Membranes upon Poration.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Probability and kinetics of rupture and electrofusion in giant unilamellar vesicles under various frequencies of direct current pulses.PloS one · 2024Article
- Differential regulation of GUV mechanics via actin network architectures.Biophysical journal · 2023Article
- Repair of traumatic lesions to the plasmalemma of neurons and other cells: Commonalities, conflicts, and controversies.Frontiers in physiology · 2023Review
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Methods to mechanically perturb and characterize GUV-based minimal cell models.Computational and structural biotechnology journal · 2023Review
- GM1 asymmetry in the membrane stabilizes pores.Biophysical journal · 2022Article
- Effects of sugar concentration on the electroporation, size distribution and average size of charged giant unilamellar vesicles.European biophysics journal : EBJ · 2022Article
- Manufacturing polymeric porous capsules.Chemical communications (Cambridge, England) · 2022Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fast digital imaging was used to study the deformation and poration of giant unilamellar vesicles subjected to electric pulses. For the first time the dynamics of response and relaxation of the membrane at micron-scale level is revealed at a time resolution of 30 micros. Above a critical transmembrane potential the lipid bilayer ruptures. Formation of macropores (diameter approximately 2 microm) with pore lifetime of approximately 10 ms has been detected. The pore lifetime has been interpreted as interplay between the pore edge tension and the membrane viscosity. The reported data, covering six decades of time, show the following regimes in the relaxation dynamics of the membrane. Tensed vesicles first relax to release the acquired stress due to stretching, approximately 100 micros. In the case of poration, membrane resealing occurs with a characteristic time of approximately 10 ms. Finally, for vesicles with excess area an additional slow regime was observed, approximately 1 s, which we associate with relaxation of membrane curvature. Dimensional analysis can reasonably well explain the corresponding characteristic timescales. Being performed on cell-sized giant unilamellar vesicles, this study brings insight to cell electroporation. The latter is widely used for gene transfection and drug transport across the membrane where processes occurring at different timescales may influence the efficiency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.