ReviewAnnals of botany2005
Plant DNA flow cytometry and estimation of nuclear genome size.
Review in Annals of botany, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 268 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
268 citing papers in PubMed.
- A pseudo-phased genome assembly for Hemileia vastatrix reveals an isolate-specific chromosomal haploid trisomy.G3 (Bethesda, Md.) · 2026Article
- Linking variation in genome size to cyst size in chrysophytes.Journal of phycology · 2026Article
- Nuclear DNA Amount in a Diverse Collection ofInternational journal of molecular sciences · 2026Article
- Nuclear diversity in Charophyceae revealed by fluorescence staining and its implications for genome size estimation.Planta · 2026Article
- Geographically proximate rare species exhibit strong population divergence while maintaining intraspecific genetic diversity in Homoranthus (Myrtaceae).Annals of botany · 2026Article
- Evolution and diversification of PAL-mediated salicylic acid biosynthesis in Rosaceae.Horticulture research · 2026Article
- Nuclear genome profiling of two species ofNAR genomics and bioinformatics · 2026Article
- Pangenome and resequencing analyses reveal flowering evolution and genetic control in Cerasus.Nature communications · 2026Article
- Agro-Morphological and Cytogenetic Characterization ofPlants (Basel, Switzerland) · 2026Article
- Fruit and seed attributes of plants derived from crossing between triploid Passionflower cytotypes.Journal of plant research · 2026Article
- Chromosome-level genome assembly of Rhynchium brunneum (Fabricius, 1793) (Hymenoptera: Vespidae).Scientific data · 2026Article
- Molecular profiling of 35 Phacelia species using SCoT molecular markers and genome size estimation.Journal of applied genetics · 2026Article
- Intraspecific genome size variation is attributed to adaptive silencing of transposable elements in Hordeum species.Molecular biology and evolution · 2026Article
- Nuclear DNA Content Analyses by Flow Cytometry of Saffron (Current issues in molecular biology · 2026Article
- Induction of Synthetic Apomixis in Two Sorghum Hybrids Enables Seed Yield and Genotype Preservation Over Multiple Generations.Plant biotechnology journal · 2026Article
- An intraspecific origin of B chromosomes in Tetragonisca fiebrigi (Apidae: Meliponini) inferred from cytogenetic and nuclear genome size data.Scientific reports · 2026Article
- Phylotranscriptomics and genome-size evidence clarify the Taiwanese Cirsium japonicum complex and delimit C. brevicaule and allied East Asian thistles.BMC plant biology · 2026Article
- Multi-dimensional identification and quality evaluation ofFrontiers in plant science · 2026Article
- The different routes of parallel evolution in epiarenic growth in a hyperarid desert environment.Frontiers in plant science · 2026Article
- Flow Cytometry and K-mer-Based Approach for Genome Size Estimation of Clusterbean: A Case Study.Methods in molecular biology (Clifton, N.J.) · 2026Article
208 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDNA flow cytometry describes the use of flow cytometry for estimation of DNA quantity in cell nuclei. The method involves preparation of aqueous suspensions of intact nuclei whose DNA is stained using a DNA fluorochrome. The nuclei are classified according to their relative fluorescence intensity or DNA content. Because the sample preparation and analysis is convenient and rapid, DNA flow cytometry has become a popular method for ploidy screening, detection of mixoploidy and aneuploidy, cell cycle analysis, assessment of the degree of polysomaty, determination of reproductive pathway, and estimation of absolute DNA amount or genome size. While the former applications are relatively straightforward, estimation of absolute DNA amount requires special attention to possible errors in sample preparation and analysis. SCOPE: The article reviews current procedures for estimation of absolute DNA amounts in plants using flow cytometry, with special emphasis on preparation of nuclei suspensions, stoichiometric DNA staining and the use of DNA reference standards. In addition, methodological pitfalls encountered in estimation of intraspecific variation in genome size are discussed as well as problems linked to the use of DNA flow cytometry for fieldwork.
conclusionsReliable estimation of absolute DNA amounts in plants using flow cytometry is not a trivial task. Although several well-proven protocols are available and some factors controlling the precision and reproducibility have been identified, several problems persist: (1) the need for fresh tissues complicates the transfer of samples from field to the laboratory and/or their storage; (2) the role of cytosolic compounds interfering with quantitative DNA staining is not well understood; and (3) the use of a set of internationally agreed DNA reference standards still remains an unrealized goal.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.