ArticleBiochemical and biophysical research communications2005
Kinetic characteristics of native gamma-glutamylcysteine ligase in the aging housefly, Musca domestica L.
Article in Biochemical and biophysical research communications, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Effects of GSH1 and GSH2 Gene Mutation on Glutathione Synthetases Activity of Saccharomyces cerevisiae.The protein journal · 2017Article
- The influence of pro-longevity gene Gclc overexpression on the age-dependent changes in Drosophila transcriptome and biological functions.BMC genomics · 2016Article
- Oxidative stress response and Nrf2 signaling in aging.Free radical biology & medicine · 2015Review
- Aging alters circadian regulation of redox in Drosophila.Frontiers in genetics · 2015Article
- Phospholipase A2 - nexus of aging, oxidative stress, neuronal excitability, and functional decline of the aging nervous system? Insights from a snail model system of neuronal aging and age-associated memory impairment.Frontiers in genetics · 2014Review
- Circadian regulation of glutathione levels and biosynthesis in Drosophila melanogaster.PloS one · 2012Article
- Association between life-span extension by caloric restriction and thiol redox state in two different strains of mice.Free radical biology & medicine · 2011Article
- Early onset senescence occurs when fibroblasts lack the glutamate-cysteine ligase modifier subunit.Free radical biology & medicine · 2009Article
- Age-associated perturbations in glutathione synthesis in mouse liver.The Biochemical journal · 2007Article
- Mechanisms of gamma-glutamylcysteine ligase regulation.Biochimica et biophysica acta · 2006Article
- Differential regulation of glutamate-cysteine ligase subunit expression and increased holoenzyme formation in response to cysteine deprivation.The Biochemical journal · 2006Article
- Pro-oxidant shift in glutathione redox state during aging.Advanced drug delivery reviewsReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The catalytic activity of gamma-glutamylcysteine ligase (gamma-GCL; EC 6.3.2.2) was compared between relatively young (4-day-old) and old (19-day-old) houseflies (Musca domestica) in order to understand the mechanism of putative deterioration of glutathione homeostasis during the aging process. Hanes-Woolf analyses ([S]/v vs [S]) indicated that gamma-GCL had significantly higher affinities for its substrates in the young than in the old flies. The K(m) values in the young and old flies were, respectively, for glutamate 0.6 and 5.5 mM; for cysteine 0.3 and 4.6 mM; and for ATP 1.2 and 2.9 mM. Furthermore, young but not old flies exhibited substrate-dependent inhibition of gamma-GCL activity at >5 mM cysteine indicating a loss of metabolic regulation during aging. The age-associated differences in the affinity of native gamma-GCL towards its substrates suggest that de novo synthesis of glutathione would be relatively less efficient in the old houseflies.
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