ArticleGut2005
Human peripheral and gastric lymphocyte responses to Helicobacter pylori NapA and AphC differ in infected and uninfected individuals.
Article in Gut, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 35 citations in OpenAlex.
- CD4European journal of medical research · 2024Article
- Review
- Gut-brain Axis and migraine headache: a comprehensive review.The journal of headache and pain · 2020Review
- Helicobacter pylori-Mediated Protection from Allergy Is Associated with IL-10-Secreting Peripheral Blood Regulatory T Cells.Frontiers in immunology · 2016Article
- Role of Toll-like receptors in Helicobacter pylori infection and immunity.World journal of gastrointestinal pathophysiology · 2014Review
- Investigation of interleukin-10 promoter polymorphisms and interleukin-10 levels in children with irritable bowel syndrome.Gut and liver · 2013Article
- The immunomodulatory properties of Helicobacter pylori confer protection against allergic and chronic inflammatory disorders.Frontiers in cellular and infection microbiology · 2012Review
- Lymphocyte proliferative response to Helicobacter pylori antigens in H. pylori-infected patients.Folia microbiologica · 2010Article
- Production and characterization of mouse monoclonal antibodies recognizing multiple subclasses of human IgG.Avicenna journal of medical biotechnology · 2010Article
- Generation and Characterization of Mouse Hybridomas Secreting Monoclonal Antibodies Specific for Human IgG3.Avicenna journal of medical biotechnology · 2009Article
Corrections and comments
- Erratum issuedMorales, VA [corrected to Athie-Morales, V]
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn this study, we identify the nature of the immunological response of human peripheral blood mononuclear cells (PBMC) and lamina propria gastric lymphocytes (LPL) to two Helicobacter pylori antigens, the neutrophil activating protein (NapA) and alkyl hydroperoxide reductase (AphC). These antigens were identified and selected for study based on the observation that serological recognition of these proteins was associated with H pylori negative status in humans.
aimsThe aim was to study the serological, proliferative, and cytokine responses of PBMC and LPL, obtained from H pylori infected and uninfected individuals, to these antigens.
methodsPatient serum, PBMC, and LPL were used to determine antibody isotype, and proliferative and cytokine responses to recombinant forms of NapA and AphC using western blotting and ELISA.
resultsWestern blotting revealed antibody reactivity to recombinant NapA and AphC among the H pylori negative population studied. Both the proliferative and interferon gamma responses of PBMC and LPL to NapA and AphC were significantly higher in H pylori negative compared with H pylori positive subjects. Analysis of the IgG subclass profiles to both antigens revealed a T helper 1 associated IgG3 antibody response in uninfected individuals. However, interleukin 10 production was greater in H pylori positive individuals in response to these antigens.
conclusionsTaken together these data are consistent with an immune response to these antigens skewed towards a T helper 1 response in the uninfected cohort.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.