ArticleProceedings of the National Academy of Sciences of the United States of America2004
Vesicular reuptake inhibition by a synaptotagmin I C2B domain antibody at the squid giant synapse.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Sensing Exocytosis and Triggering Endocytosis at Synapses: Synaptic Vesicle Exocytosis-Endocytosis Coupling.Frontiers in cellular neuroscience · 2018Review
- Developmental changes in Ca2+ channel subtypes regulating endocytosis at the calyx of Held.The Journal of physiology · 2014Article
- Early and progressive impairment of spinal blood flow-glucose metabolism coupling in motor neuron degeneration of ALS model mice.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2012Article
- Expression, localization, and functional role for synaptotagmins in pancreatic acinar cells.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Synaptotagmin IV acts as a multi-functional regulator of Ca2+-dependent exocytosis.Neurochemical research · 2011Review
- Developmental shift to a mechanism of synaptic vesicle endocytosis requiring nanodomain Ca2+.Nature neuroscience · 2010Article
- Involvement of Ca2+ channel synprint site in synaptic vesicle endocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Role of Rab27 in synaptic transmission at the squid giant synapse.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Molecular basis of synaptic vesicle cargo recognition by the endocytic sorting adaptor stonin 2.The Journal of cell biology · 2007Article
- The role of synaptotagmin I C2A calcium-binding domain in synaptic vesicle clustering during synapse formation.The Journal of physiology · 2007Article
- Distinct roles of the C2A and the C2B domain of the vesicular Ca2+ sensor synaptotagmin 9 in endocrine beta-cells.The Biochemical journal · 2007Article
- 1-Methyl-4-phenylpyridinium affects fast axonal transport by activation of caspase and protein kinase C.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- 1-Methyl-4-phenylpyridinium induces synaptic dysfunction through a pathway involving caspase and PKCdelta enzymatic activities.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- C2B polylysine motif of synaptotagmin facilitates a Ca2+-independent stage of synaptic vesicle priming in vivo.Molecular biology of the cell · 2006Article
- Synaptic transmission at retinal ribbon synapses.Progress in retinal and eye research · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptotagmin (Syt) I, a ubiquitous synaptic vesicle protein, comprises a transmembrane region and two C2 domains. The C2 domains, which have been shown to be essential for both synaptic vesicle exocytosis and endocytosis, are also seen as the Ca(2+) sensors in synaptic vesicular release. In a previous study, we reported that a polyclonal antibody raised against the squid (Loligo pealei) Syt I C2B domain, while inhibiting vesicular endocytosis, was synaptic release neutral at the squid giant synapse. Recent reports concerning the C2B requirements for synaptic release prompted us to readdress the role of C2B in squid giant synapse function. Presynaptic injection of another anti-Syt I-C2B antibody (using recombinant whole C2B domain expressed in mammalian cell culture as an antigen) into the presynaptic terminal reproduced our previous results, i.e., reduction of vesicular endocytosis without affecting synaptic release. This set of results addresses the issue of the geometrical arrangement of the Ca(2+) sensor, allowing the C2B domain antibody to restrict Ca(2+)-dependent C2B self-oligomerization without modifying the Ca(2+)-dependent release process.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.