ArticleMolecular biology of the cell2005
Nm23H2 facilitates coat protein complex II assembly and endoplasmic reticulum export in mammalian cells.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- IRF6 Regulates the Delivery of E-Cadherin to the Plasma Membrane.The Journal of investigative dermatology · 2022Article
- NDK/NME proteins: a host-pathogen interface perspective towards therapeutics.Current genetics · 2022Review
- The Subcellular Localization and Oligomerization Preferences of NME1/NME2 upon Radiation-Induced DNA Damage.International journal of molecular sciences · 2020Article
- Article
- The TRAPP complex mediates secretion arrest induced by stress granule assembly.The EMBO journal · 2019Article
- Regulation of heterotrimeric G-protein signaling by NDPK/NME proteins and caveolins: an update.Laboratory investigation; a journal of technical methods and pathology · 2018Review
- Nucleoside diphosphate kinase B regulates angiogenic responses in the endothelium via caveolae formation and c-Src-mediated caveolin-1 phosphorylation.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2017Article
- Progress on Nme (NDP kinase/Nm23/Awd) gene family-related functions derived from animal model systems: studies on development, cardiovascular disease, and cancer metastasis exemplified.Naunyn-Schmiedeberg's archives of pharmacology · 2015Article
- Alterations in reversible protein histidine phosphorylation as intracellular signals in cardiovascular disease.Frontiers in pharmacology · 2015Review
- PIWIL2 induces c-Myc expression by interacting with NME2 and regulates c-Myc-mediated tumor cell proliferation.Oncotarget · 2014Article
- Regulation of traffic and organelle architecture of the ER-Golgi interface by signal transduction.Histochemistry and cell biology · 2013Review
- Identification of discrete sites in Yip1A necessary for regulation of endoplasmic reticulum structure.PloS one · 2013Article
- Nucleoside-diphosphate-kinase: a pleiotropic effector in microbial colonization under interdisciplinary characterization.Microbes and infection · 2012Review
- Proteomic analysis of NME1/NDPK A null mouse liver: evidence for a post-translational regulation of annexin IV and EF-1Bα.Naunyn-Schmiedeberg's archives of pharmacology · 2011Article
- Learning about the functions of NME/NM23: lessons from knockout mice to silencing strategies.Naunyn-Schmiedeberg's archives of pharmacology · 2011Review
- Nucleoside diphosphate kinase B is required for the formation of heterotrimeric G protein containing caveolae.Naunyn-Schmiedeberg's archives of pharmacology · 2011Article
- Article
- MAPK signaling to the early secretory pathway revealed by kinase/phosphatase functional screening.The Journal of cell biology · 2010Article
- Yip1A structures the mammalian endoplasmic reticulum.Molecular biology of the cell · 2010Article
- Requirements for transitional endoplasmic reticulum site structure and function in Saccharomyces cerevisiae.Molecular biology of the cell · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytosolic coat protein complex II (COPII) mediates vesicle formation from the endoplasmic reticulum (ER) and is essential for ER-to-Golgi trafficking. The minimal machinery for COPII assembly is well established. However, additional factors may regulate the process in mammalian cells. Here, a morphological COPII assembly assay using purified COPII proteins and digitonin-permeabilized cells has been applied to demonstrate a role for a novel component of the COPII assembly pathway. The factor was purified and identified by mass spectrometry as Nm23H2, one of eight isoforms of nucleoside diphosphate kinase in mammalian cells. Importantly, recombinant Nm23H2, as well as a catalytically inactive version, promoted COPII assembly in vitro, suggesting a noncatalytic role for Nm23H2. Consistent with a function for Nm23H2 in ER export, Nm23H2 localized to a reticular network that also stained for the ER marker calnexin. Finally, an in vivo role for Nm23H2 in COPII assembly was confirmed by isoform-specific knockdown of Nm23H2 by using short interfering RNA. Knockdown of Nm23H2, but not its most closely related isoform Nm23H1, resulted in diminished COPII assembly at steady state and reduced kinetics of ER export. These results strongly suggest a previously unappreciated role for Nm23H2 in mammalian ER export.
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