ArticleNucleic acids research2004
A novel E2 box-GATA element modulates Cdc6 transcription during human cells polyploidization.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Enhancement of PRMT6 binding to a novel germlineHaematologica · 2024Article
- E2A Modulates Stemness, Metastasis, and Therapeutic Resistance of Breast Cancer.Cancer research · 2021Article
- CDC6 expression is regulated by lineage-specific transcription factor GATA1.Cell cycle (Georgetown, Tex.) · 2012Article
- Inferring transcription factor complexes from ChIP-seq data.Nucleic acids research · 2011Article
- Endoreplication: polyploidy with purpose.Genes & development · 2009Review
- Coordinated activation of the origin licensing factor CDC6 and CDK2 in resting human fibroblasts expressing SV40 small T antigen and cyclin E.The Journal of biological chemistry · 2009Article
- Mechanisms to control rereplication and implications for cancer.Current opinion in cell biology · 2007Review
- The SCL +40 enhancer targets the midbrain together with primitive and definitive hematopoiesis and is regulated by SCL and GATA proteins.Molecular and cellular biology · 2007Article
- Identification of genes targeted by the androgen and PKA signaling pathways in prostate cancer cells.Oncogene · 2006Article
- Article
- Endomitosis: a new cell fate in the cell cycle leading to polyploidy in megakaryocytes and hepatocytes.Journal of Zhejiang University. Science. BReview
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cdc6 is a key regulator of the strict alternation of S and M phases during the mitotic cell cycle. In mammalian and plant cells that physiologically become polyploid, cdc6 is transcriptionally and post-translationally regulated. We have recently reported that Cdc6 levels are maintained in megakaryoblastic HEL cells, but severely downregulated by ectopic expression of transcriptional repressor Drosophila melanogaster escargot. Here, we show that cdc6 promoter activity is upregulated during megakaryocytic differentiation of HEL endoreplicating cells, and that Escargot interferes with such activation. Transactivation experiments showed that a 1.7 kb region located at 2800 upstream cdc6 transcription initiation site behaved as a potent enhancer in endoreplicating cells only. This activity was mainly dependent on a novel cis-regulatory element composed by an E2 box overlapping a GATA motif. Ectopic Escargot could bind this regulatory element in vitro and endogenous GATA-1 and E2A formed specific complexes in megakaryoblastic cells as well as in primary megakaryocytes. Chromatin Immunoprecipitation analysis revealed that both transcription factors were occupying the E2 box/GATA site in vivo. Altogether, these data suggest that cdc6 expression could be actively maintained during megakaryocytic differentiation through transcriptional mechanisms involving specific cis- and trans-regulatory elements.
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