ArticleEukaryotic cell2004
Prm1 prevents contact-dependent lysis of yeast mating pairs.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
- The chitin synthase regulator CSR-3 promotes cellular integrity during cell-cell fusion in the filamentous ascomycete fungus Neurospora crassa.PLoS genetics · 2025Article
- Identification of regions required for allelic specificity at the cell wall remodeling allorecognition checkpoint in Neurospora crassa.Genetics · 2025Article
- Live imaging-based assay for visualising species-specific interactions in gamete adhesion molecules.Scientific reports · 2022Article
- Plasma Membrane Fusion Is Specifically Impacted by the Molecular Structure of Membrane Sterols During Vegetative Development ofGenetics · 2020Article
- The molecular foundations of zygosis.Cellular and molecular life sciences : CMLS · 2020Review
- Article
- Cell fusion in yeast is negatively regulated by components of the cell wall integrity pathway.Molecular biology of the cell · 2019Article
- Identification and characterization of LFD-2, a predicted fringe protein required for membrane integrity during cell fusion in neurospora crassa.Eukaryotic cell · 2015Article
- Membrane organization and cell fusion during mating in fission yeast requires multipass membrane protein Prm1.Genetics · 2014Article
- Genetic basis of cell-cell fusion mechanisms.Trends in genetics : TIG · 2013Review
- Mate and fuse: how yeast cells do it.Open biology · 2013Review
- Activation of an essential calcium signaling pathway in Saccharomyces cerevisiae by Kch1 and Kch2, putative low-affinity potassium transporters.Eukaryotic cell · 2013Article
- Cdc42p and Fus2p act together late in yeast cell fusion.Molecular biology of the cell · 2012Article
- Prm1 targeting to contact sites enhances fusion during mating in Saccharomyces cerevisiae.Eukaryotic cell · 2010Article
- The yeast cell fusion protein Prm1p requires covalent dimerization to promote membrane fusion.PloS one · 2010Article
- Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Prm1 functions as a disulfide-linked complex in yeast mating.The Journal of biological chemistry · 2010Article
- Lipid mixing and content release in single-vesicle, SNARE-driven fusion assay with 1-5 ms resolution.Biophysical journal · 2009Article
- Article
- Membrane lysis during biological membrane fusion: collateral damage by misregulated fusion machines.The Journal of cell biology · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane fusion requires localized destabilization of two phospholipid bilayers, but unrestrained membrane destabilization could result in lysis. prm1 mutant yeast cells have a defect at the plasma membrane fusion stage of mating that typically results in the accumulation of prezygotes that have fingers of membrane-bound cytoplasm projecting from one cell of each pair into its mating partner in the direction of the osmotic gradient between the cells. However, some prm1 mating pairs fuse successfully whereas the two cells in other prm1 mating pairs simultaneously lyse. Lysis only occurs if both mating partners are prm1 mutants. Osmotic stabilization does not protect prm1 mating pairs from lysis, indicating that lysis is not caused by a cell wall defect. prm1 mating pairs without functional mitochondria still lyse, ruling out programmed cell death. No excess lysis was found after pheromone treatment of haploid prm1 cells, and lysis did not occur in mating pairs when prm1 was combined with the fus1 and fus2 mutations to block cell wall remodeling. Furthermore, short (<1 microm) cytoplasmic microfingers indicating the completion of cell wall remodeling appeared immediately before lysis. In combination, these results demonstrate that plasma membrane contact is a prerequisite for lysis. Cytoplasmic microfingers are unlikely to cause lysis since most prm1 mating pairs with microfingers do not lyse, and microfingers were also detected before fusion in some wild-type mating pairs. The lysis of prm1 mutant mating pairs suggests that the Prm1 protein stabilizes the membrane fusion event of yeast mating.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.