ArticleEukaryotic cell2004
UGD1, encoding the Cryptococcus neoformans UDP-glucose dehydrogenase, is essential for growth at 37 degrees C and for capsule biosynthesis.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 70 citations in OpenAlex.
- Pathogenic diversity ofCell surface (Amsterdam, Netherlands) · 2025Article
- Elevated UDP-glucuronic acid levels mend drug resistance and stress responses via a protease and a transporter inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Organoselenium Has a Potent Fungicidal Effect on Cryptococcus neoformans and Inhibits the Virulence Factors.Antimicrobial agents and chemotherapy · 2023Article
- Influence of Pathogen Carbon Metabolism on Interactions With Host Immunity.Frontiers in cellular and infection microbiology · 2022Review
- Genetic analysis of electroactive biofilms.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Moderate levels of 5-fluorocytosine cause the emergence of high frequency resistance in cryptococci.Nature communications · 2021Article
- Article
- Review
- The cell wall proteome from two strains of Pseudocercospora fijiensis with differences in virulence.World journal of microbiology & biotechnology · 2019Article
- The cAMP/Protein Kinase a Pathway Regulates Virulence and Adaptation to Host Conditions inFrontiers in cellular and infection microbiology · 2019Review
- Article
- Unraveling synthesis of the cryptococcal cell wall and capsule.Glycobiology · 2018Review
- Cryptococcus neoformans can form titan-like cells in vitro in response to multiple signals.PLoS pathogens · 2018Article
- Article
- Xylose donor transport is critical for fungal virulence.PLoS pathogens · 2018Article
- The environmental yeast Cryptococcus liquefaciens produces capsular and secreted polysaccharides with similar pathogenic properties to those of C. neoformans.Scientific reports · 2017Article
- Comparative Ecology of CapsularFrontiers in microbiology · 2017Article
- Masking the Pathogen: Evolutionary Strategies of Fungi and Their Bacterial Counterparts.Journal of fungi (Basel, Switzerland) · 2015Review
- Article
- Model-driven mapping of transcriptional networks reveals the circuitry and dynamics of virulence regulation.Genome research · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
We report the identification and disruption of the Cryptococcus neoformans var. grubii UGD1 gene encoding the UDP-glucose dehydrogenase, which catalyzes the conversion of UDP-glucose into UDP-glucuronic acid. Deletion of UGD1 led to modifications in the cell wall, as revealed by changes in the sensitivity of ugd1Delta cells to sodium dodecyl sulfate, NaCl, and sorbitol. Moreover, two of the yeast's major virulence factors-capsule biosynthesis and the ability to grow at 37 degrees C-were impaired in ugd1Delta strains. These results suggest that the UDP-dehydrogenase represents the major, and maybe only, biosynthetic pathway for UDP-glucuronic acid in C. neoformans. Consequently, deletion of UGD1 blocked not only the synthesis of UDP-glucuronic acid but also that of UDP-xylose. To differentiate the phenotype(s) associated with the UDP-glucuronic acid defect alone from those linked to the UDP-xylose defect, ugd1Delta mutants were phenotypically compared to strains from which the gene encoding UDP-xylose synthase (i.e., that required for synthesis of UDP-xylose) had been deleted. Finally, studies of strains from which one of the four CAP genes (CAP10, CAP59, CAP60, or CAP64) had been deleted revealed common cell wall phenotypes associated with the acapsular state.
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