ArticleEukaryotic cell2004
The histone fold domain of Cse4 is sufficient for CEN targeting and propagation of active centromeres in budding yeast.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.Frontiers in cell and developmental biology · 2026Review
- The centromeric histone CenH3 is recruited into the tombusvirus replication organelles.PLoS pathogens · 2022Article
- Centromere Engineering as an Emerging Tool for Haploid Plant Production: Advances and Challenges.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Skp, Cullin, F-box (SCF)-Met30 and SCF-Cdc4-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A for Chromosomal Stability in Budding Yeast.PLoS genetics · 2020Article
- Conformational flexibility of histone variant CENP-AThe Journal of biological chemistry · 2018Article
- Architecture of the CBF3-centromere complex of the budding yeast kinetochore.Nature structural & molecular biology · 2018Article
- A Genome-Wide Screen Reveals a Role for the HIR Histone Chaperone Complex in Preventing Mislocalization of Budding Yeast CENP-A.Genetics · 2018Article
- Connecting GCN5's centromeric SAGA to the mitotic tension-sensing checkpoint.Molecular biology of the cell · 2018Article
- The Ubiquitin Ligase (E3) Psh1p Is Required for Proper Segregation of both Centromeric and Two-Micron Plasmids inG3 (Bethesda, Md.) · 2017Article
- A novel role of the N terminus of budding yeast histone H3 variant Cse4 in ubiquitin-mediated proteolysis.Genetics · 2013Article
- Phosphorylation of the CENP-A amino-terminus in mitotic centromeric chromatin is required for kinetochore function.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The ABCs of CENPs.Chromosoma · 2011Review
- The rapidly evolving centromere-specific histone has stringent functional requirements in Arabidopsis thaliana.Genetics · 2010Article
- Cse4 is part of an octameric nucleosome in budding yeast.Molecular cell · 2009Article
- CENPA a genomic marker for centromere activity and human diseases.Current genomics · 2009Article
- Altered dosage and mislocalization of histone H3 and Cse4p lead to chromosome loss in Saccharomyces cerevisiae.Genetics · 2008Article
- Structure, dynamics, and evolution of centromeric nucleosomes.Proceedings of the National Academy of Sciences of the United States of America · 2007Review
- Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Phylogenetic analysis of fungal centromere H3 proteins.Genetics · 2006Article
- The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation.The Journal of cell biology · 2006Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Centromere-specific H3-like proteins (CenH3s) are conserved across the eukaryotic kingdom and are required for packaging centromere DNA into a specialized chromatin structure required for kinetochore assembly. Cse4 is the CenH3 protein of the budding yeast Saccharomyces cerevisiae. Like all CenH3 proteins, Cse4 consists of a conserved histone fold domain (HFD) and a divergent N terminus (NT). The Cse4 NT contains an essential domain designated END (for essential N-terminal domain); deletion of END is lethal. To investigate the role of the Cse4 NT in centromere targeting, a series of deletion alleles (cse4DeltaNT) were analyzed. No part of the Cse4 NT was required to target mutant proteins to centromere DNA in the presence of functional Cse4. A Cse4 degron strain was used to examine targeting of a Cse4DeltaNT protein in the absence of wild-type Cse4. The END was not required for centromere targeting under these conditions, confirming that the HFD confers specificity of Cse4 centromere targeting. Surprisingly, overexpression of the HFD bypassed the requirement for the END altogether, and viable S. cerevisiae strains in which the cells express only the Cse4 HFD and six adjacent N-terminal amino acids (Cse4Delta129) were constructed. Despite the complete absence of the NT, mitotic chromosome loss in the cse4Delta129 strain increased only 6-fold compared to a 15-fold increase in strains overexpressing wild-type Cse4. Thus, when overexpressed, the Cse4 HFD is sufficient for centromere function in S. cerevisiae, and no posttranslational modification or interaction of the NT with other kinetochore component(s) is essential for accurate chromosome segregation in budding yeast.
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