ReviewJournal of the American Society for Mass Spectrometry2004
Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry.
Review in Journal of the American Society for Mass Spectrometry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Unexpected Reduction of Iminoquinone and Quinone Derivatives in Positive Electrospray Ionization Mass Spectrometry and Possible Mechanism Exploration.Journal of the American Society for Mass Spectrometry · 2017Article
- Oxidative Ionization Under Certain Negative-Ion Mass Spectrometric Conditions.Journal of the American Society for Mass Spectrometry · 2017Article
- A Disposable Microfluidic Device with a Screen Printed Electrode for Mimicking Phase II Metabolism.Sensors (Basel, Switzerland) · 2016Article
- Characterization of the Cysteine Content in Proteins Utilizing Cysteine Selenylation with 266 nm Ultraviolet Photodissociation (UVPD).Journal of the American Society for Mass Spectrometry · 2016Article
- Investigation of some biologically relevant redox reactions using electrochemical mass spectrometry interfaced by desorption electrospray ionization.Analytical and bioanalytical chemistry · 2012Article
- Oxidative protein labeling in mass-spectrometry-based proteomics.Analytical and bioanalytical chemistry · 2010Review
- Site-selective fragmentation of peptides and proteins at quinone-modified cysteine residues investigated by ESI-MS.Analytical chemistry · 2010Article
- On-line electrogeneration of copper-peptide complexes in microspray mass spectrometry.Journal of the American Society for Mass Spectrometry · 2008Article
- Mass spectrometric methods for monitoring redox processes in electrochemical cells.Mass spectrometry reviewsReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present herein a review of our work on the on-line electrochemical generation of mass tags toward cysteine residues in peptides and proteins. Taking advantage of the inherent electrochemical nature of electrospray generated from a microfabricated microspray emitter, selective probes for cysteine were developed and tested for on-line nonquantitative mass tagging of peptides and proteins. The nonquantitative aspect of the covalent tagging thus allows direct counting of free cysteines in the mass spectrum of a biomolecule through additional adduct peaks. Several substituted hydroquinones were investigated in terms of electrochemical properties, and their usefulness for on-line mass tagging during microspray experiments were assessed with L-cysteine, peptides, and intact proteins. Complementarily, numerical simulations were performed to properly understand the respective roles of mass transport, kinetics of electrochemical-chemical reactions, and design of the microspray emitter in the mass tagging overall efficiency. Finally, the on-line electrochemical tagging of cysteine residues was applied to the analysis of tryptic peptides of purified model proteins for protein identification through peptide mass fingerprinting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.