ArticleNucleic acids research2004
Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Dissecting the development of bovine testicular tissue using spatial transcriptomics.Journal of animal science and biotechnology · 2026Article
- A missense mutation in TCFL5 is associated with male infertility due to oligoasthenoteratozoospermia.Molecular genetics and genomics : MGG · 2025Article
- The MYBL1/TCFL5 transcription network: two collaborative factors with central role in male meiosis.Biochemical Society transactions · 2023Review
- Proximity Proteomics Revealed Aberrant mRNA Splicing Elicited by ALS-Linked Profilin-1 Mutants.Analytical chemistry · 2023Article
- Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch.Cell death & disease · 2023Article
- The transcription factor TCFL5 responds to A-MYB to elaborate the male meiotic program in mice.Reproduction (Cambridge, England) · 2023Article
- Article
- TCFL5 deficiency impairs the pachytene to diplotene transition during spermatogenesis in the mouse.Scientific reports · 2022Article
- Isoform-specific effects of transcription factor TCFL5 on the pluripotency-related genes SOX2 and KLF4 in colorectal cancer development.Molecular oncology · 2022Article
- The upregulation ofFrontiers in oncology · 2022Article
- Pseudoautosomal Region 1 Overdosage Affects the Global Transcriptome in iPSCs From Patients With Klinefelter Syndrome and High-Grade X Chromosome Aneuploidies.Frontiers in cell and developmental biology · 2021Article
- Gestational arsenic exposure induces site-specific DNA hypomethylation in active retrotransposon subfamilies in offspring sperm in mice.Epigenetics & chromatin · 2020Article
- Transcriptomic analysis of female and male gonads in juvenile snakeskin gourami (Trichopodus pectoralis).Scientific reports · 2020Article
- Comparative expression profiling of testis-enriched genes regulated during the development of spermatogonial cells.PloS one · 2017Article
- Article
- An integrative genomic analysis of the superior fecundity phenotype in QSi5 mice.Molecular biotechnology · 2013Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Regulation of tissue-specific expression of SPATULA, a bHLH gene involved in carpel development, seedling germination, and lateral organ growth in Arabidopsis.Journal of experimental botany · 2010Article
- Tagged mutagenesis by efficient Minos-based germ line transposition.Molecular and cellular biology · 2010Article
- Genomic expression profiling of mature soybean (Glycine max) pollen.BMC plant biology · 2009Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mouse spermatogenesis, differentiating germ line cells initiate expression of specific genes at subsequent developmental steps. The Calmegin (Clgn) gene is first expressed in meiotic prophase, in primary spermatocytes, and encodes a protein that acts as a chaperone. To identify testis-specific transcription factors that control expression of the Clgn gene in spermatogenesis, we performed a yeast one-hybrid screening with a Clgn promoter sequence as bait DNA. This screening resulted in the identification of mouse Tcfl5 as a candidate Clgn promoter-binding protein. Tcfl5 is a member of the basic helix-loop-helix (bHLH) family of transcription factors, and mouse Tcfl5 shows 83% amino acid sequence identity with human TCFL5. Gel-shift and yeast one-hybrid experiments showed that Tcfl5 interacts with a non-canonical CACGCG site that is present in the Clgn promoter. By using northern blot, RT-PCR and in situ hybridization, mouse Tcfl5 mRNA was detected only in testis, with the highest expression level in primary spermatocytes and round spermatids. The highest level of Tcfl5 protein was found in primary spermatocytes at the diplotene stage of meiotic prophase, where the protein colocalizes with transcriptionally active chromatin.
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