ArticleJournal of clinical microbiology2004
Comparison of five methods for extraction of Legionella pneumophila from respiratory specimens.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Evaluation of Factors that Affect the Performance of COVID-19 Molecular Assays Including Presence of Symptoms, Number of Detected Genes and RNA Extraction Type.Molecular diagnosis & therapy · 2022Article
- A Comparison of Five SARS-CoV-2 Molecular Assays With Clinical Correlations.American journal of clinical pathology · 2021Article
- Direct Clinical Evidence Recommending the Use of Proteinase K or Dithiothreitol to Pretreat Sputum for Detection of SARS-CoV-2.Frontiers in medicine · 2020Article
- Comparative Evaluation of Three Preprocessing Methods for Extraction and Detection of Influenza A Virus Nucleic Acids from Sputum.Frontiers in medicine · 2018Article
- Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR.Annals of laboratory medicine · 2016Article
- Alternative molecular tests for virological diagnosis.Molecular biotechnology · 2013Review
- Laboratory detection of respiratory viruses by automated techniques.The open virology journal · 2012Article
- Comparison of three rapid and easy bacterial DNA extraction methods for use with quantitative real-time PCR.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Rapid detection of blaKPC carbapenemase genes by internally controlled real-time PCR assay using bactec blood culture bottles.Journal of clinical microbiology · 2011Article
- Comparison of commercial systems for extraction of nucleic acids from DNA/RNA respiratory pathogens.Journal of virological methods · 2011Article
- Multicenter comparative evaluation of five commercial methods for toxoplasma DNA extraction from amniotic fluid.Journal of clinical microbiology · 2009Article
- Comparison of the sensitivity of culture, PCR and quantitative real-time PCR for the detection of Pseudomonas aeruginosa in sputum of cystic fibrosis patients.BMC microbiology · 2009Article
- Comparison of automated nucleic acid extraction methods with manual extraction.The Journal of molecular diagnostics : JMD · 2008Article
- Quantitative real-time PCR is not more sensitive than "conventional" PCR.Journal of clinical microbiology · 2008Article
- Currently used nucleic acid amplification tests for the detection of viruses and atypicals in acute respiratory infections.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2007Review
- Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples.Journal of clinical microbiology · 2007Article
- Evaluation of NucliSens easyMAG for automated nucleic acid extraction from various clinical specimens.Journal of clinical microbiology · 2007Article
- Evaluation of molecular diagnostic assays for fungal infections.The Journal of molecular diagnostics : JMD · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The efficiencies of five commercially available nucleic acid extraction methods were evaluated for the recovery of a standardized inoculum of Legionella pneumophila in respiratory specimens (sputum and bronchoalveolar lavage [BAL] specimens). The concentrations of Legionella DNA recovered from sputa with the automated MagNA Pure (526,200 CFU/ml) and NucliSens (171,800 CFU/ml) extractors were greater than those recovered with the manual methods (i.e., Roche High Pure kit [133,900 CFU/ml], QIAamp DNA Mini kit [46,380 CFU/ml], and ViralXpress kit [13,635 CFU/ml]). The rank order was the same for extracts from BAL specimens, except that for this specimen type the QIAamp DNA Mini kit recovered more than the Roche High Pure kit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.