ArticleJournal of clinical microbiology2004
Evaluation of multiplex PCRs for diagnosis of infection with diarrheagenic Escherichia coli and Shigella spp.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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Who cites it
90 citing papers in PubMed.
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- Virulence and Antibiotic Resistance of aEPEC/STECPathogens (Basel, Switzerland) · 2025Article
- The diverse virulence potential of atypical enteropathogenicFrontiers in microbiology · 2025Article
- Diarrhoeagenic Escherichia coli associated with childhood diarrhoea in Osun state, Nigeria.BMC infectious diseases · 2024Article
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- Suboptimal Bacteriological Quality of Household Water in Municipal Ibadan, Nigeria.The American journal of tropical medicine and hygiene · 2024Article
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- Gut microbiota composition in travellers is associated with faecal lipocalin-2, a mediator of gut inflammation.Frontiers in cellular and infection microbiology · 2024Article
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- Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of EnterotoxigenicMicroorganisms · 2023Article
- Resistance and Virulence Surveillance inMicroorganisms · 2023Article
- Prevalence and Antimicrobial Resistance Profile of DiarrheagenicAntibiotics (Basel, Switzerland) · 2023Article
30 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We have developed two multiplex PCR assays that detect typical and atypical enteropathogenic Escherichia coli (EPEC) isolates, enteroaggregative E. coli (EAEC) isolates, enterotoxigenic E. coli (ETEC) isolates, enteroinvasive E. coli (EIEC) isolates, Shiga toxin-producing E. coli (STEC) isolates, and Shigella spp. The targets selected for each group were eae and bfpA for EPEC isolates, the target of probe CVD432 for EAEC isolates, the genes encoding heat-labile and heat-stable toxins for ETEC isolates, stx(1) and stx(2) for STEC isolates, and ipaH for EIEC isolates and Shigella spp. These PCRs were specific and sensitive for rapid detection of target isolates in stools. Among 150 stool specimens from the acute diarrhea tested, 9 samples (6%) had atypical EPEC, 9 (6%) had typical EPEC, 7 (4.7%) had EAEC, 3 (2%) had EIEC, 3 (2%) had Shigella spp., and 1 (0.7%) had an O26 STEC strain; we also detected mixed infections, 2 (1.3%) with EAEC and Shigella spp., 1 (0.7%) with atypical and typical EPEC strains, and another with atypical EPEC and EAEC strains. One of the multiplex PCRs directly applied to 36 stool specimens correctly identified 100% of EPEC and EAEC isolates.
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