ArticleJournal of clinical microbiology2004
Real-time PCR assay for a unique chromosomal sequence of Bacillus anthracis.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Implications of limits of detection of various methods for Bacillus anthracis in computing risks to human health.Applied and environmental microbiology · 2009Pooled it
- Terbium Phenanthroline Complex as a Luminescent Probe for the Detection of Anthrax Biomarker: Dipicolinic acid.Journal of fluorescence · 2025Article
- Anti-infective nitazoxanide disrupts transcription of ribosome biogenesis-related genes in yeast.Genes & genomics · 2020Article
- Evaluation of Real-time PCR Based on SYBR Green I Fluorescent Dye for Detection ofJournal of veterinary research · 2018Article
- In silico and in vitro evaluation of PCR-based assays for the detection of Bacillus anthracis chromosomal signature sequences.Virulence · 2013Article
- Article
- Use of a bacteriophage lysin to identify a novel target for antimicrobial development.PloS one · 2013Article
- High-throughput screening of a diversity collection using biodefense category A and B priority pathogens.Journal of biomolecular screening · 2012Article
- Development of aptamer beacons for rapid presumptive detection of Bacillus spores.Journal of fluorescence · 2012Article
- The genome and variation of Bacillus anthracis.Molecular aspects of medicine · 2009Review
- Gamma irradiation can be used to inactivate Bacillus anthracis spores without compromising the sensitivity of diagnostic assays.Applied and environmental microbiology · 2008Article
- High throughput screening for spores and vegetative forms of pathogenic B. anthracis by an internally controlled real-time PCR assay with automated DNA preparation.Medical microbiology and immunology · 2007Article
- The Bacillus anthracis chromosome contains four conserved, excision-proficient, putative prophages.BMC microbiology · 2006Article
- Requirements for the Development of Bacillus Anthracis Spore Reference Materials Used to Test Detection Systems.Journal of research of the National Institute of Standards and TechnologyArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time PCR has become an important method for the rapid identification of Bacillus anthracis since the 2001 anthrax mailings. Most real-time PCR assays for B. anthracis have been developed to detect virulence genes located on the pXO1 and pXO2 plasmids. In contrast, only two published chromosomal targets exist, the rpoB gene and the gyrA gene. In the present study, subtraction-hybridization with a plasmid-cured B. anthracis tester strain and a Bacillus cereus driver was used to find a unique chromosomal sequence. By targeting this region, a real-time assay was developed with the Ruggedized Advanced Pathogen Identification Device. Further testing has revealed that the assay has 100% sensitivity and 100% specificity, with a limit of detection of 50 fg of DNA. The results of a search for sequences with homology with the BLAST program demonstrated significant alignment to the recently published B. anthracis Ames strain, while an inquiry for protein sequence similarities indicated homology with an abhydrolase from B. anthracis strain A2012. The importance of this chromosomal assay will be to verify the presence of B. anthracis independently of plasmid occurrence.
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