ArticleJournal of clinical microbiology2004
Use of highly variable intergenic spacer sequences for multispacer typing of Rickettsia conorii strains.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 93 citations in OpenAlex.
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- Analysis of Rocky Mountain spotted fever cases in Northern Mexico reveals genetic variability of Rickettsia rickettsii and the different distribution of genotypes.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Rickettsial Infection in Ticks from a National Park in the Cerrado Biome, Midwestern Brazil.Pathogens (Basel, Switzerland) · 2023Article
- Use of eschar swab DNA to diagnoseInfectious medicine · 2023Article
- History and Current Status of Mediterranean Spotted Fever (MSF) in the Crimean Peninsula and Neighboring Regions along the Black Sea Coast.Pathogens (Basel, Switzerland) · 2023Review
- A Review of Rickettsial Diseases Other Than Scrub Typhus in India.Tropical medicine and infectious disease · 2023Review
- Molecular Evidence for Flea-Borne Rickettsiosis in Febrile Patients from Madagascar.Pathogens (Basel, Switzerland) · 2021Article
- Phylogenetic Differentiation of Rickettsia parkeri Reveals Broad Dispersal and Distinct Clustering within North American Strains.Microbiology spectrum · 2021Article
- The Potential Relevance of the Microbiome to Hair Physiology and Regeneration: The Emerging Role of Metagenomics.Biomedicines · 2021Review
- Bifidobacteria Strain Typing by Fourier Transform Infrared Spectroscopy.Frontiers in microbiology · 2021Article
- Tracking of deliberately inoculatedFood science and biotechnology · 2020Article
- Evolutionary Diversity in the Intracellular Microsporidian Parasite Nosema sp. Infecting Wild Silkworm Revealed by IGS Nucleotide Sequence Diversity.Journal of molecular evolution · 2020Article
- Isolation and characterization of a Rickettsia from the ovary of a Western black-legged tick, Ixodes pacificus.Ticks and tick-borne diseases · 2019Article
- Phylogenetic Evidence for the Existence of Multiple Strains of Rickettsia parkeri in the New World.Applied and environmental microbiology · 2018Article
- Genotypic Characterization ofBioMed research international · 2018Article
- Molecular surveillance of spotted fever group rickettsioses in wildlife and detection ofThe Onderstepoort journal of veterinary research · 2017Article
- Extremely Low Genomic Diversity of Rickettsia japonica Distributed in Japan.Genome biology and evolution · 2017Article
- Development and Validation of an Improved PCR Method Using the 23S-5S Intergenic Spacer for Detection of Rickettsiae in Dermacentor variabilis Ticks and Tissue Samples from Humans and Laboratory Animals.Journal of clinical microbiology · 2016Article
- Rickettsia rickettsii in Amblyomma patinoi ticks, Colombia.Emerging infectious diseases · 2015Article
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Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By use of the nearly perfectly colinear genomes of Rickettsia conorii and Rickettsia prowazekii, we compared the usefulness of three types of sequences for typing of R. conorii isolates: (i) 5 variable coding genes comprising the 16S ribosomal DNA, gltA, ompB, and sca4 (gene D) genes, which are present in both genomes, and the ompA gene, which is degraded in R. prowazekii; (ii) 28 genes degraded in R. conorii but intact in R. prowazekii, including 23 split and 5 remnant genes; and (iii) 27 conserved and 25 variable intergenic spacers. The 4 conserved and 23 split genes as well as the 27 conserved intergenic spacers each had identical sequences in 34 human and 5 tick isolates of R. conorii. Analysis of the ompA sequences identified three genotypes of R. conorii. The variable intergenic spacers were significantly more variable than conserved genes, split genes, remnant genes, and conserved spacers (P < 10(-2) in all cases). Four of the variable intergenic spacers (dksA-xerC, mppA-purC, rpmE-tRNA(fMet), and tRNA(Gly)-tRNA(Tyr)) had highly variable sequences; when they were combined for typing, multispacer typing (MST) identified 27 different genotypes in the 39 R. conorii isolates. Two batches from the same R. conorii strain, Malish (Seven), with different culture passage histories were found to exhibit the same MST type. MST was more discriminatory for strain genotyping than multiple gene sequencing (P < 10(-2)). Phylogenetic analysis based on MST sequences was concordant with the geographic origins of R. conorii isolates. Our study supports the usefulness of MST for strain genotyping. This tool may be useful for tracing a strain and identifying its source during outbreaks, including those resulting from bioterrorism.
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