ArticleJournal of clinical microbiology2004
Simultaneous detection of Staphylococcus aureus and coagulase-negative staphylococci in positive blood cultures by real-time PCR with two fluorescence resonance energy transfer probe sets.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02850653 (Contribution of Fast Molecular Bacterial Identification by Real-time PCR in Managing of Postoperative Acute Endophthalmitis), which is not on this map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Contribution of Fast Molecular Bacterial Identification by Real-time PCR in Managing of Postoperative Acute Endophthalmitis
Who cites it
25 citing papers in PubMed, 78 citations in OpenAlex.
- Combinatorial efficacy of Manuka honey and antibiotics in theFrontiers in cellular and infection microbiology · 2023Article
- Development and Evaluation of Duplex MIRA-qPCR Assay for Simultaneous Detection of Staphylococcus aureus and non-aureus StaphylococciMicroorganisms · 2022Article
- Quantification of Bacteria in Mouth-Rinsing Solution for the Diagnosis of Periodontal Disease.Journal of clinical medicine · 2021Article
- Propidium monoazide-polymerase chain reaction for detection of residual periprosthetic joint infection in two-stage revision.Molecular biology reports · 2019Article
- Accuracy of PCR targeting different markers for Staphylococcus aureus identification: a comparative study using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as the gold standard.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
- Evaluation of HiaureusJournal of clinical and diagnostic research : JCDR · 2017Article
- Usefulness of Multiplex Real-Time PCR for Simultaneous Pathogen Detection and Resistance Profiling of Staphylococcal Bacteremia.BioMed research international · 2016Article
- Evaluation of a Genus- and Group-Specific Rapid PCR Assay Panel on Synovial Fluid for Diagnosis of Prosthetic Knee Infection.Journal of clinical microbiology · 2016Article
- Rapid identification of Staphylococcus aureus directly from Bactec blood culture broth by the BinaxNOW S. aureus test.Journal of clinical microbiology · 2014Article
- Rapid molecular microbiologic diagnosis of prosthetic joint infection.Journal of clinical microbiology · 2013Article
- Thymidine auxotrophic Staphylococcus aureus small-colony variant endocarditis and left ventricular assist device infection.Journal of clinical microbiology · 2012Article
- Methicillin-resistant coagulase-negative staphylococci on pig farms as a reservoir of heterogeneous staphylococcal cassette chromosome mec elements.Applied and environmental microbiology · 2012Article
- Comparison of three rapid and easy bacterial DNA extraction methods for use with quantitative real-time PCR.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Acceleration of the direct identification of Staphylococcus aureus versus coagulase-negative staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Dabigatran inhibits Staphylococcus aureus coagulase activity.Journal of clinical microbiology · 2010Article
- The usefulness of multiplex PCR for the identification of bacteria in joint infection.Journal of clinical laboratory analysis · 2010Article
- Irinotecan-induced mucositis manifesting as diarrhoea corresponds with an amended intestinal flora and mucin profile.International journal of experimental pathology · 2009Article
- Microbiologic diagnosis of prosthetic shoulder infection by use of implant sonication.Journal of clinical microbiology · 2009Article
- Molecular identification of bacteria from aseptically loose implants.Clinical orthopaedics and related research · 2008Article
- Population genetic structure of the Staphylococcus intermedius group: insights into agr diversification and the emergence of methicillin-resistant strains.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time PCR assay that uses two fluorescence resonance energy transfer probe sets and targets the tuf gene of staphylococci is described here. One probe set detects the Staphylococcus genus, whereas the other probe set is specific for Staphylococcus aureus. One hundred thirty-eight cultured isolates, which contained 41 isolates of staphylococci representing at least nine species, and 100 positive blood cultures that contained gram-positive cocci in clusters were tested. This assay was 100% sensitive and 100% specific for the detection of the Staphylococcus genus and of S. aureus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.