ArticleJournal of clinical microbiology2004
Phylogenetic analysis and PCR-restriction fragment length polymorphism identification of Campylobacter species based on partial groEL gene sequences.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 63 citations in OpenAlex.
- Polyphasic Characterisation ofMicroorganisms · 2020Article
- Campylobacter portucalensis sp. nov., a new species of Campylobacter isolated from the preputial mucosa of bulls.PloS one · 2020Article
- Minimal standards for describing new species belonging to the families Campylobacteraceae and Helicobacteraceae: Campylobacter, Arcobacter, Helicobacter and Wolinella spp.International journal of systematic and evolutionary microbiology · 2017Article
- Cleaning and disinfection programs against Campylobacter jejuni for broiler chickens: productive performance, microbiological assessment and characterization.Poultry science · 2017Article
- Novel Sampling Method for Assessing Human-Pathogen Interactions in the Natural Environment Using Boot Socks and Citizen Scientists, with Application to Campylobacter Seasonality.Applied and environmental microbiology · 2017Article
- Isolation of Campylobacter spp. from Three Species of Antarctic Penguins in Different Geographic Locations.EcoHealth · 2017Article
- Comprehensive genomic characterization of campylobacter genus reveals some underlying mechanisms for its genomic diversification.PloS one · 2013Article
- Updated 16S rRNA-RFLP method for the identification of all currently characterised Arcobacter spp.BMC microbiology · 2012Article
- Campylobacter troglodytis sp. nov., isolated from feces of human-habituated wild chimpanzees (Pan troglodytes schweinfurthii) in Tanzania.Applied and environmental microbiology · 2011Article
- The analysis of groEL gene in Salmonella enterica subspecies enterica serovar Typhimurium isolated from avians by PCR-Restriction Fragment Length Polymorphism method.Veterinary research communications · 2011Article
- Comparison of Arcobacter isolation methods, and diversity of Arcobacter spp. in Cheshire, United Kingdom.Applied and environmental microbiology · 2011Article
- Taxonomy, epidemiology, and clinical relevance of the genus Arcobacter.Clinical microbiology reviews · 2011Review
- Isolation of a novel Campylobacter jejuni clone associated with the bank vole, Myodes glareolus.Applied and environmental microbiology · 2010Article
- Use of groESL as a target for identification of Abiotrophia, Granulicatella, and Gemella species.Journal of clinical microbiology · 2010Article
- Rapid evolution and the importance of recombination to the gastroenteric pathogen Campylobacter jejuni.Molecular biology and evolution · 2009Article
- Species-specific identification of Campylobacters by PCR-restriction fragment length polymorphism and PCR targeting of the gyrase B gene.Applied and environmental microbiology · 2008Article
- Atypical Helicobacter canadensis strains associated with swine.Applied and environmental microbiology · 2006Article
- Genetic divergence of Campylobacter fetus strains of mammal and reptile origins.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phylogeny of 12 Campylobacter species and reference strains of Arcobacter butzleri and Helicobacter pylori was studied based on partial 593-bp groEL gene sequences. The topology of the phylogenetic neighbor-joining tree based on the groEL gene was similar to that of the tree based on the 16S rRNA gene. However, groEL was found to provide a better resolution for Campylobacter species, with lower interspecies sequence similarities (range, 65 to 94%) compared with those for the 16S rRNA gene (range, 90 to 99%) and high intraspecies sequence similarities (range, 95 to 100%; average, 99%). A new universal reverse primer that amplifies a 517-bp fragment of the groEL gene was developed and used for PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of 68 strains representing 11 Campylobacter species as well as reference strains of A. butzlerii and H. pylori. Digestion with the AluI enzyme discriminated all Campylobacter species included in the study but showed more intraspecies diversity than digestion with the ApoI enzyme. A hippurate-negative variant of Campylobacter jejuni with a high level of groEL sequence similarity to both C. jejuni (96%) and C. coli (94%) gave a unique AluI profile and an ApoI profile identical to those of other C. jejuni strains. In conclusion, groEL gene sequencing and PCR-RFLP analysis are recommended as valuable tools for the identification of Campylobacter species.
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