ArticleJournal of clinical microbiology2004
Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 288 papers, 3 of them syntheses that pooled it.
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Who cites it
288 citing papers in PubMed, 3 syntheses or guidelines pooled it, 462 citations in OpenAlex.
- Transfusion-transmitted malaria in sub-Saharan Africa: a systematic review and meta-analysis of Plasmodium spp. carriage in blood units and confirmed transmission in recipient.Malaria journal · 2026Pooled it
- Overreliance on Plasmodium 18S rRNA gene for malaria molecular diagnosis-inferences from systematic review.Malaria journal · 2025Pooled it
- Misidentification of Plasmodium ovale as Plasmodium vivax malaria by a microscopic method: a meta-analysis of confirmed P. ovale cases.Scientific reports · 2020Pooled it
- Effect of mass drug administration on malaria incidence in southeast Senegal during 2020-22: a two-arm, open-label, cluster-randomised controlled trial.The Lancet. Infectious diseases · 2025Trial
- Feasibility and safety of integrating mass drug administration for helminth control with seasonal malaria chemoprevention among Senegalese children: a randomized controlled, observer-blind trial.Malaria journal · 2023Trial
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- Trial
- Comparative permissiveness of Anopheles stephensi colony mosquito with an Anopheles arabiensis colony to Plasmodium falciparum gametocytes in Metehara, Ethiopia.Parasites & vectors · 2026Article
- Integration of vector and human behavior in residual malaria in rural communities in the Peruvian Amazon.PLoS neglected tropical diseases · 2026Article
- Detection ofASM case reports · 2026Article
- Severe Plasmodium falciparum infection with multi-organ involvement in an asplenic patient: a case report.Malaria journal · 2026Article
- Performance of High-Resolution Melting Analysis for Molecular Detection and Species Differentiation of Malaria: A Scoping Review.Pathogens (Basel, Switzerland) · 2026Article
- Genome-wide characterization of Plasmodium vivax infections in local travelers and non-travelers from the Peruvian Amazon.Research square · 2026Article
- Prevalence and risk factors of asymptomatic Plasmodium spp. infection in the military population of the Colombian National Army.PLoS neglected tropical diseases · 2026Article
- Malaria among febrile patients during a yellow fever outbreak in Ghana: a cross-sectional study.Malaria journal · 2026Article
- Article
- Genetic diversity of the Plasmodium falciparum msp2 gene in children from the Eastern Democratic Republic of Congo: a comparative study of clinical and subclinical infections.BMC medical genomics · 2026Article
- Malaria Risk among Internally Mobile Individuals and Heterogeneous Mobility Patterns in Two Hypoendemic Communities: Implications for Malaria Elimination in the Peruvian Amazon.medRxiv : the preprint server for health sciences · 2026Article
- Accurate and sensitive TaqMan real-time PCR assays to detect and quantify simian malaria parasites Plasmodium cynomolgi, Plasmodium inui and Plasmodium coatneyi.Malaria journal · 2026Article
- Ten years of Plasmodium malariae infections in an International Health Reference Center in Barcelona: temporal trends and insights.Malaria journal · 2026Observational
228 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There have been reports of increasing numbers of cases of malaria among migrants and travelers. Although microscopic examination of blood smears remains the "gold standard" in diagnosis, this method suffers from insufficient sensitivity and requires considerable expertise. To improve diagnosis, a multiplex real-time PCR was developed. One set of generic primers targeting a highly conserved region of the 18S rRNA gene of the genus Plasmodium was designed; the primer set was polymorphic enough internally to design four species-specific probes for P. falciparum, P. vivax, P. malarie, and P. ovale. Real-time PCR with species-specific probes detected one plasmid copy of P. falciparum, P. vivax, P. malariae, and P. ovale specifically. The same sensitivity was achieved for all species with real-time PCR with the 18S screening probe. Ninety-seven blood samples were investigated. For 66 of them (60 patients), microscopy and real-time PCR results were compared and had a crude agreement of 86% for the detection of plasmodia. Discordant results were reevaluated with clinical, molecular, and sequencing data to resolve them. All nine discordances between 18S screening PCR and microscopy were resolved in favor of the molecular method, as were eight of nine discordances at the species level for the species-specific PCR among the 31 samples positive by both methods. The other 31 blood samples were tested to monitor the antimalaria treatment in seven patients. The number of parasites measured by real-time PCR fell rapidly for six out of seven patients in parallel to parasitemia determined microscopically. This suggests a role of quantitative PCR for the monitoring of patients receiving antimalaria therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.