ArticleJournal of clinical microbiology2004
Practical implementation of a multiplex PCR for acute respiratory tract infections in children.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 62 citations in OpenAlex.
- Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome.Microorganisms · 2022Review
- Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia.Journal of virological methods · 2019Article
- The use of multiplex PCR for the diagnosis of viral severe acute respiratory infection in children: a high rate of co-detection during the winter season.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Is there still room for novel viral pathogens in pediatric respiratory tract infections?PloS one · 2014Article
- Validation of a multiplex reverse transcriptase PCR ELISA for the detection of 19 respiratory tract pathogens.Infection · 2013Article
- Viral agents causing lower respiratory tract infections in hospitalized children: evaluation of the Speed-Oligo® RSV assay for the detection of respiratory syncytial virus.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- [Children with cancer and respiratory viral infection: epidemiology, diagnosis and treatment options].Enfermedades infecciosas y microbiologia clinica · 2011Review
- Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Review
- Respiratory syncytial virus and human rhinoviruses are the major causes of severe lower respiratory tract infections in Kuwait.Journal of medical virology · 2010Article
- Performance of diagnostic tests to detect respiratory viruses in older adults.Diagnostic microbiology and infectious disease · 2010Article
- Utilization of nucleic acid amplification assays for the detection of respiratory viruses.Clinics in laboratory medicine · 2009Review
- Identification of respiratory viruses in adults: nasopharyngeal versus oropharyngeal sampling.Journal of clinical microbiology · 2009Article
- Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children.Journal of clinical microbiology · 2009Article
- Optimal sampling sites and methods for detection of pathogens possibly causing community-acquired lower respiratory tract infections.Journal of clinical microbiology · 2009Review
- [Viral respiratory tract infections in the first six months of life].Anales de pediatria (Barcelona, Spain : 2003) · 2008Article
- Detection of respiratory viruses by molecular methods.Clinical microbiology reviews · 2008Review
- Utility of DNA microarrays for detection of viruses in acute respiratory tract infections in children.The Journal of pediatrics · 2008Article
- Development of real-time PCR assays for detection and quantification of human bocavirus.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2008Article
- Multiplex real-time PCR for detection of respiratory tract infections.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2008Article
- [Virology: the contribution of molecular biology in the microbiologic diagnosis of pediatric respiratory diseases].Archives de pediatrie : organe officiel de la Societe francaise de pediatrie · 2007Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular testing for acute respiratory infections (ARIs) has documented value but limited implementation due to questions that typically slow the acceptance of new tests. This study sought to address these questions and achieve implementation. Rhinovirus was added to a nested multiplex PCR (M-PCR), increasing its diagnostic yield. Over one winter, three hospital pediatric departments used the M-PCR to complement their direct fluorescent-antibody assay (DFA) for respiratory syncytial virus (RSV). Clinicians recorded "pretest probability estimates" (using continuous scales for various pathogen groups) for comparison with test results; treatments and test turnaround times were also recorded. Transnasal and throat swabs, with or without nasopharyngeal aspirate (NPA), were M-PCR tested. NPA-containing sample sets found to be RSV positive by DFA were not further tested. Single PCR for human metapneumovirus (hMPV) was performed retrospectively. Of 178 ARI episodes representing 172 patients, NPA was included in 97 sample sets; 54 (56%) were determined to be RSV positive. The other NPA-containing sample sets (n = 43) yielded 27 findings (63%), and the swab-only sets (n = 81) yielded 47 findings (58%); rhinovirus was found most often. Testing for hMPV yielded seven positive results. M-PCR median turnaround times were 4 days in swab-only samples and 5 days with NPA. Antibiotics were prescribed in 50 episodes, at rates similar for RSV and rhinovirus. Pretest probability estimates of a viral cause were lower in episodes caused by rhinovirus than in episodes caused by RSV. The hospitals continued to use M-PCR for NPA-containing samples found to be RSV negative by DFA. Test implementation is more likely with higher diagnostic yield and a protocol that reflects day-to-day clinical and laboratory operations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.