ArticleJournal of clinical microbiology2004
Pulsed-field gel electrophoresis study of Mycobacterium abscessus isolates previously affected by DNA degradation.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 77 citations in OpenAlex.
- Open-Label Trial of Amikacin Liposome Inhalation Suspension in Mycobacterium abscessus Lung Disease.Chest · 2023Article
- Efficacy of three sputum specimens for the diagnosis of Mycobacterium avium complex pulmonary disease.BMC pulmonary medicine · 2023Review
- DNA Phosphorothioate Modifications Are Widely Distributed in the Human Microbiome.Biomolecules · 2020Article
- Phosphorothioated DNA Is Shielded from Oxidative Damage.Applied and environmental microbiology · 2019Article
- DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function.Scientific reports · 2017Article
- Two-Phase Hospital-Associated Outbreak of Mycobacterium abscessus: Investigation and Mitigation.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- Multilocus sequence typing scheme versus pulsed-field gel electrophoresis for typing Mycobacterium abscessus isolates.Journal of clinical microbiology · 2014Article
- Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences.Nature communications · 2014Article
- Clonal relationship and differentiation among Mycobacterium abscessus isolates as determined using the semiautomated repetitive extragenic palindromic sequence PCR-based DiversiLab system.Journal of clinical microbiology · 2014Article
- Current methods in the molecular typing of Mycobacterium tuberculosis and other mycobacteria.BioMed research international · 2014Review
- Phosphorothioate DNA as an antioxidant in bacteria.Nucleic acids research · 2012Article
- Five-year outbreak of community- and hospital-acquired Mycobacterium porcinum infections related to public water supplies.Journal of clinical microbiology · 2011Article
- Mycobacterium lentiflavum in drinking water supplies, Australia.Emerging infectious diseases · 2011Article
- Multilocus sequence analysis and rpoB sequencing of Mycobacterium abscessus (sensu lato) strains.Journal of clinical microbiology · 2011Article
- Conditional gene expression in Mycobacterium abscessus.PloS one · 2011Article
- Twenty years hunting for sulfur in DNA.Protein & cell · 2010Review
- Inaccuracy of single-target sequencing for discriminating species of the Mycobacterium abscessus group.Journal of clinical microbiology · 2009Article
- Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii.Journal of clinical microbiology · 2009Article
- Non mycobacterial virulence genes in the genome of the emerging pathogen Mycobacterium abscessus.PloS one · 2009Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA degradation (which results in a smear pattern) occurs with almost 50% of Mycobacterium abscessus strains during pulsed-field gel electrophoresis (PFGE). We assessed the potential benefit of using thiourea-containing buffer with M. abscessus by studying 69 isolates not previously typeable by PFGE (i.e., those with a smear pattern). Random (epidemiologically unrelated) isolates that were typeable (no smear pattern) were included as controls. Genomic DNA was digested with DraI, XbaI, and AseI. PFGE gels were run in regular gel buffer with and without 100 muM thiourea. All 69 isolates that generated smear patterns had clear band profiles when the thiourea buffer was used. These isolates were divided into only 30 patterns with DraI, 20 patterns with XbaI, and 20 patterns with AseI. The molecular profiles were all closely or possibly related, and the differences between the isolates ranged from zero to six bands. By multilocus enzyme electrophoresis (MEE), 45 of 53 smear isolates (85%) belonged to two closely related electrophoretic types. These isolates contained at least one enzyme allele seen almost exclusively in this group. Isolates without smear patterns were unaffected by thiourea and produced unrelated PFGE profiles, as well as multiple MEE types. The hsp65 and 16S rRNA gene sequences of the isolates with smear patterns were identical to those of M. abscessus type strain ATCC 19977, which had a nonsmear pattern, suggesting that this clone is a subgroup within M. abscessus. This demonstrates that the inability to type M. abscessus by PFGE is associated with a single clone of organisms.
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