ArticleJournal of clinical microbiology2004
Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
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- An evaluation of the impact of abattoir processing on the levels ofFrontiers in microbiology · 2025Article
- Uncovering the boundaries ofmSystems · 2024Article
- Genomic and clinical characteristics of campylobacteriosis in Australia.Microbial genomics · 2024Article
- Genotyping and antibiotic susceptibility of Campylobacter species isolated from raw milk samples in Qazvin, Iran.BMC research notes · 2023Article
- Investigating the Significance of Non-Healthcare (Basel, Switzerland) · 2023Article
- Foodborne Pathogens at the Livestock-Wildlife-Human Interface in Rural Western Uganda.EcoHealth · 2023Article
- Etiology and risk factors for diarrheal disease amongst rural and peri-urban populations in Cambodia, 2012-2018.PloS one · 2023Article
- Weaning transition, but not the administration of probiotic candidateFrontiers in veterinary science · 2023Article
- Description of novel capsule biosynthesis loci of Campylobacter jejuni clinical isolates from South and South-East Asia.PloS one · 2023Article
- Molecular Epidemiological Evidence Implicates Cattle as a Primary Reservoir ofPathogens (Basel, Switzerland) · 2022Article
- Should We Consider Them as a Threat? Antimicrobial Resistance, Virulence Potential and Genetic Diversity ofAntibiotics (Basel, Switzerland) · 2022Article
- Socio-ecological Factors of Zoonotic Diseases Exposure in Colorado Dairy Workers.Journal of agromedicine · 2021Article
- Guillain-Barré Syndrome Outbreak in Peru 2019 Associated WithNeurology(R) neuroimmunology & neuroinflammation · 2021Article
- Assessing the role of livestock and sympatric wild ruminants in spreading antimicrobial resistant Campylobacter and Salmonella in alpine ecosystems.BMC veterinary research · 2021Article
- Analysis of Campylobacter jejuni Subtype Distribution in the Chicken Broiler Production Continuum: a Longitudinal Examination To Identify Primary Contamination Points.Applied and environmental microbiology · 2021Article
- Association of Broiler Litter Microbiome Composition andFrontiers in veterinary science · 2021Article
- Vaccines against gastroenteritis, current progress and challenges.Gut microbes · 2020Review
- Whole genome sequencing reveals extended natural transformation in Campylobacter impacting diagnostics and the pathogens adaptive potential.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expression plasmid that restored wild-type lipopolysaccharide levels in Escherichia coli strain SM105 [lpxA(Ts)]. With oligonucleotide primers developed to the C. jejuni lpxA gene, nearly full-length lpxA amplicons were amplified from an additional 11 isolates of C. jejuni, 20 isolates of C. coli, 16 isolates of C. lari, and five isolates of C. upsaliensis. The nucleotide sequence of each amplicon was determined, and sequence alignment revealed a high level of species discrimination. Oligonucleotide primers were constructed to exploit species differences, and a multiplex PCR assay was developed to positively identify isolates of C. coli, C. jejuni, C. lari, and C. upsaliensis. We characterized an additional set of 41 thermotolerant isolates by partial nucleotide sequence analysis to further demonstrate the uniqueness of each species-specific region. The multiplex PCR assay was validated with 105 genetically defined isolates of C. coli, C. jejuni, C. lari, and C. upsaliensis, 34 strains representing 12 additional Campylobacter species, and 24 strains representing 19 non-Campylobacter species. Application of the multiplex PCR method to whole-cell lysates obtained from 108 clinical and environmental thermotolerant Campylobacter isolates resulted in 100% correlation with biochemical typing methods.
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