ArticleGenetics2004
Gene deletions by ends-in targeting in Drosophila melanogaster.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed.
- Article
- Homologous recombination is essential for DNA damage-induced regeneration of Drosophila testis germline stem cells.Development (Cambridge, England) · 2026Article
- Single-cell transcriptomics of X-ray irradiatedeLife · 2026Article
- Distinct p53 isoforms code for opposing transcriptional outcomes.Developmental cell · 2022Article
- Article
- Identification and Characterization of Breakpoints and Mutations onG3 (Bethesda, Md.) · 2020Article
- Article
- Probing the Function of Metazoan Histones with a Systematic Library of H3 and H4 Mutants.Developmental cell · 2019Article
- p53 is required for brain growth but is dispensable for resistance to nutrient restriction during Drosophila larval development.PloS one · 2018Article
- Article
- Roles for the Histone Modifying and Exchange Complex NuA4 in Cell Cycle Progression in Drosophila melanogaster.Genetics · 2016Article
- Sir2 Acts through Hepatocyte Nuclear Factor 4 to maintain insulin Signaling and Metabolic Homeostasis in Drosophila.PLoS genetics · 2016Article
- The function of Drosophila p53 isoforms in apoptosis.Cell death and differentiation · 2015Article
- Corp Regulates P53 in Drosophila melanogaster via a Negative Feedback Loop.PLoS genetics · 2015Article
- Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase β and regulates complex V activity.The Journal of cell biology · 2014Article
- Strategies for gene disruption in Drosophila.Cell & bioscience · 2014Review
- Accelerated homologous recombination and subsequent genome modification in Drosophila.Development (Cambridge, England) · 2013Article
- Cre-mediated targeted gene activation in the middle silk glands of transgenic silkworms (Bombyx mori).Transgenic research · 2013Article
- The border between the ultrabithorax and abdominal-A regulatory domains in the Drosophila bithorax complex.Genetics · 2013Article
- A SIRT1-LSD1 corepressor complex regulates Notch target gene expression and development.Molecular cell · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Following the advent of a gene targeting technique in Drosophila, different methods have been developed to modify the Drosophila genome. The initial demonstration of gene targeting in flies used an ends-in method, which generates a duplication of the target locus. The duplicated locus can then be efficiently reduced to a single copy by generating a double-strand break between the duplicated segments. This method has been used to knock out target genes by introducing point mutations. A derivative of this method is reported here. By using different homologous regions for the targeting and reduction steps, a complete deletion of the target gene can be generated to produce a definitive null allele. The breakpoints of the deletion can be precisely controlled. Unlike ends-out targeting, this method does not leave exogenous sequence at the deleted locus. Three endogenous genes, Sir2, Sirt2, and p53 have been successfully deleted using this method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.