ArticleGenetics2004
The enhancer-blocking activity of the Fab-7 boundary from the Drosophila bithorax complex requires GAGA-factor-binding sites.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 105 citations in OpenAlex.
- Chromatin insulators in gene regulation and 3D genome organization.Biochemical Society transactions · 2025Review
- Article
- Enhancer-promoter interactions become more instructive in the transition from cell-fate specification to tissue differentiation.Nature genetics · 2024Article
- Temporal inhibition of chromatin looping and enhancer accessibility during neuronal remodeling.Nature communications · 2021Article
- GAGA factor: a multifunctional pioneering chromatin protein.Cellular and molecular life sciences : CMLS · 2021Review
- Homeotic Genes: Clustering, Modularity, and Diversity.Frontiers in cell and developmental biology · 2021Review
- Mutational analysis identifies functional Rap1, Su(Hw), and CTCF insulator sites in Arabidopsis thaliana.Plant cell reports · 2020Article
- Interactome of vertebrate GAF/ThPOK reveals its diverse functions in gene regulation and DNA repair.Journal of biosciences · 2020Article
- BEN-solo factors partition active chromatin to ensure proper gene activation in Drosophila.Nature communications · 2019Article
- Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex.PLoS genetics · 2018Article
- The BEN Domain Protein Insensitive Binds to theGenetics · 2018Article
- The bithorax complex iab-7 Polycomb response element has a novel role in the functioning of the Fab-7 chromatin boundary.PLoS genetics · 2018Article
- Vertebrate GAF/ThPOK: emerging functions in chromatin architecture and transcriptional regulation.Cellular and molecular life sciences : CMLS · 2018Review
- High-resolution TADs reveal DNA sequences underlying genome organization in flies.Nature communications · 2018Article
- Drosophila Dosage Compensation Loci Associate with a Boundary-Forming Insulator Complex.Molecular and cellular biology · 2017Article
- Architectural protein Pita cooperates with dCTCF in organization of functional boundaries in Bithorax complex.Development (Cambridge, England) · 2017Article
- Different Evolutionary Strategies To Conserve Chromatin Boundary Function in the Bithorax Complex.Genetics · 2017Article
- The GAGA factor regulatory network: Identification of GAGA factor associated proteins.PloS one · 2017Article
- Transcription factors GAF and HSF act at distinct regulatory steps to modulate stress-induced gene activation.Genes & development · 2016Article
- Functional Dissection of the Blocking and Bypass Activities of the Fab-8 Boundary in the Drosophila Bithorax Complex.PLoS genetics · 2016Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the work reported here we have analyzed the role of the GAGA factor [encoded by the Trithorax-like (Trl) gene] in the enhancer-blocking activity of Frontabdominal-7 (Fab-7), a domain boundary element from the Drosophila melanogaster bithorax complex (BX-C). One of the three nuclease hypersensitive sites in the Fab-7 boundary, HS1, contains multiple consensus-binding sequences for the GAGA factor, a protein known to be involved in the formation and/or maintenance of nucleosome-free regions of chromatin. GAGA protein has been shown to localize to the Fab-7 boundary in vivo, and we show that it recognizes sequences from HS1 in vitro. Using two different transgene assays we demonstrate that GAGA-factor-binding sites are necessary but not sufficient for full Fab-7 enhancer-blocking activity. We show that distinct GAGA sites are required for different enhancer-blocking activities at different stages of development. We also show that the enhancer-blocking activity of the endogenous Fab-7 boundary is sensitive to mutations in the gene encoding the GAGA factor Trithorax-like.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.