ArticleGenetics2004
White mutants of Chlamydomonas reinhardtii are defective in phytoene synthase.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 92 citations in OpenAlex.
- A quick-to-implement and optimized CRISPR-Cas9 protocol to obtain insertional and small indel mutants inMethodsX · 2025Article
- Distribution, Biosynthesis, and Function of Carotenoids in Oxygenic Phototrophic Algae.Marine drugs · 2025Review
- Illuminating the diversity of carotenoids in microalgal eyespots and phototaxis.Plant signaling & behavior · 2023Review
- Zeaxanthin is required for eyespot formation and phototaxis in Euglena gracilis.Plant physiology · 2023Article
- The Chlamydomonas Genome Project, version 6: Reference assemblies for mating-type plus and minus strains reveal extensive structural mutation in the laboratory.The Plant cell · 2023Article
- Perspective: Multiomics and Machine Learning Help Unleash the Alternative Food Potential of Microalgae.Advances in nutrition (Bethesda, Md.) · 2023Article
- Channelrhodopsin-Dependent Photo-Behavioral Responses in the Unicellular Green Alga Chlamydomonas reinhardtii.Advances in experimental medicine and biology · 2021Review
- Chloroplast Sec14-like 1 (CPSFL1) is essential for normal chloroplast development and affects carotenoid accumulation inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Isolation and Characterization of Novel Chlorella Vulgaris Mutants With Low Chlorophyll and Improved Protein Contents for Food Applications.Frontiers in bioengineering and biotechnology · 2020Article
- Modification of aMethodsX · 2020Article
- Regulation of photoprotection gene expression inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus.BMC biotechnology · 2019Article
- A kiwifruit (Actinidia deliciosa) R2R3-MYB transcription factor modulates chlorophyll and carotenoid accumulation.The New phytologist · 2019Article
- Differential gene expression for carotenoid biosynthesis in a green alga Ulva prolifera based on transcriptome analysis.BMC genomics · 2018Article
- Targeting of Photoreceptor Genes inThe Plant cell · 2017Article
- Suppression of the phytoene synthase gene (EgcrtB) alters carotenoid content and intracellular structure of Euglena gracilis.BMC plant biology · 2017Article
- Complete chloroplast genomes of theMitochondrial DNA. Part B, Resources · 2017Article
- Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease.Plant physiology · 2016Article
- Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Carotenoids play an integral and essential role in photosynthesis and photoprotection in plants and algae. A collection of Chlamydomonas reinhardtii mutants lacking carotenoids was characterized for pigment and tocopherol (vitamin E) composition, growth phenotypes under different light conditions, and the molecular basis of their mutant phenotype. The carotenoid-less mutants, or "white" mutants, were also deficient in chlorophylls but had approximately twice the tocopherol content of the wild type. White mutants grew in the dark but were unable to survive in the light, even under very low light conditions on acetate-containing medium. Genetic crosses and recombination tests revealed that all individual white mutants in the collection are alleles of a single gene, lts1, and the white phenotype was closely linked to a marker located in the phytoene synthase gene. DNA sequencing of the phytoene synthase gene from each of the mutants revealed nonsense, missense, frameshift, and splice site mutations. Transformation with a wild-type copy of the phytoene synthase gene was able to complement the lts1-210 mutation. Together, these results show that all the white mutants examined in this work are affected in the phytoene synthase gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.