ArticlePlant physiology2005
NADP-malate dehydrogenase from unicellular green alga Chlamydomonas reinhardtii. A first step toward redox regulation?
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Molecular architecture of the human citrate synthase-malate dehydrogenase 2 metabolon.Acta crystallographica. Section D, Structural biology · 2026Article
- Central transcriptional regulator controls photosynthetic growth and carbon storage in response to high light.Nature communications · 2024Article
- Characterizing compensatory mechanisms in the absence of photoprotective qE in Chlamydomonas reinhardtii.Photosynthesis research · 2023Article
- Molecular Regulation and Evolution of Redox Homeostasis in Photosynthetic Machinery.Antioxidants (Basel, Switzerland) · 2022Review
- Flexibility of Oxidized and Reduced States of the Chloroplast Regulatory Protein CP12 in Isolation and in Cell Extracts.Biomolecules · 2021Article
- Turning the Knobs: The Impact of Post-translational Modifications on Carbon Metabolism.Frontiers in plant science · 2021Review
- Inhibition of TOR inCells · 2019Article
- Subcellular Energetics and Carbon Storage inCells · 2019Review
- Consequences of Oxidative Stress on Plant Glycolytic and Respiratory Metabolism.Frontiers in plant science · 2019Review
- Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas.The Plant cell · 2018Article
- Impaired Mitochondrial Transcription Termination Disrupts the Stromal Redox Poise inPlant physiology · 2017Article
- Chloroplast FBPase and SBPase are thioredoxin-linked enzymes with similar architecture but different evolutionary histories.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Comprehensively Characterizing the Thioredoxin Interactome In Vivo Highlights the Central Role Played by This Ubiquitous Oxidoreductase in Redox Control.Molecular & cellular proteomics : MCP · 2016Article
- Insight into protein S-nitrosylation in Chlamydomonas reinhardtii.Antioxidants & redox signaling · 2014Article
- Chloroplastic thioredoxin-f and thioredoxin-m1/4 play important roles in brassinosteroids-induced changes in CO2 assimilation and cellular redox homeostasis in tomato.Journal of experimental botany · 2014Article
- The yeast autophagy protease Atg4 is regulated by thioredoxin.Autophagy · 2014Article
- Identification of global ferredoxin interaction networks in Chlamydomonas reinhardtii.The Journal of biological chemistry · 2013Article
- Redox regulation of the Calvin-Benson cycle: something old, something new.Frontiers in plant science · 2013Review
- Ferredoxin:thioredoxin reductase (FTR) links the regulation of oxygenic photosynthesis to deeply rooted bacteria.Planta · 2013Article
- The chloroplastic thiol reducing systems: dual functions in the regulation of carbohydrate metabolism and regeneration of antioxidant enzymes, emphasis on the poplar redoxin equipment.Photosynthesis research · 2010Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The determinants of the thioredoxin (TRX)-dependent redox regulation of the chloroplastic NADP-malate dehydrogenase (NADP-MDH) from the eukaryotic green alga Chlamydomonas reinhardtii have been investigated using site-directed mutagenesis. The results indicate that a single C-terminal disulfide is responsible for this regulation. The redox midpoint potential of this disulfide is less negative than that of the higher plant enzyme. The regulation is of an all-or-nothing type, lacking the fine-tuning provided by the second N-terminal disulfide found only in NADP-MDH from higher plants. The decreased stability of specific cysteine/alanine mutants is consistent with the presence of a structural disulfide formed by two cysteine residues that are not involved in regulation of activity. Measurements of the ability of C. reinhardtii thioredoxin f (TRX f) to activate wild-type and site-directed mutants of sorghum (Sorghum vulgare) NADP-MDH suggest that the algal TRX f has a redox midpoint potential that is less negative than most those of higher plant TRXs f. These results are discussed from an evolutionary point of view.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.