ArticlePlanta2005
Targeted site-directed mutagenesis of a heme oxygenase locus by gene replacement in the moss Ceratodon purpureus.
Article in Planta, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Identification and Characterization of an Antifungal GeneInternational journal of molecular sciences · 2023Article
- Plastid genome analysis of three Nemaliophycidae red algal species suggests environmental adaptation for iron limited habitats.PloS one · 2018Article
- Testing of Auxotrophic Selection Markers for Use in the MossFrontiers in plant science · 2017Article
- Phytochrome-mediated regulation of cell division and growth during regeneration and sporeling development in the liverwort Marchantia polymorpha.Journal of plant research · 2015Article
- Oxylipins in moss development and defense.Frontiers in plant science · 2015Review
- High contents of very long-chain polyunsaturated fatty acids in different moss species.Plant cell reports · 2014Article
- Assembly of synthetic locked phycocyanobilin derivatives with phytochrome in vitro and in vivo in Ceratodon purpureus and Arabidopsis.The Plant cell · 2012Article
- Article
- A linkage map reveals a complex basis for segregation distortion in an interpopulation cross in the moss Ceratodon purpureus.Genetics · 2007Article
- Negative phototropic response of rhizoid cells in the fern Adiantum capillus-veneris.Journal of plant research · 2006Article
- A computational approach to discovering the functions of bacterial phytochromes by analysis of homolog distributions.BMC bioinformatics · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The moss Physcomitrella patens is so far the only plant species in which it is possible for nuclear genes to be modified by homologous recombination at a reasonably efficiency. Here we describe the use of homologous recombination for another moss, Ceratodon purpureus. Our approach is based on the repair of the ptr116 mutant allele. In this mutant, codon 31 of the heme oxygenase gene CpHO1 is mutated to a stop codon. Heme oxygenase is necessary for the conversion of heme to biliverdin, the precursor of the phytochrome chromophore. Thus, in ptr116 the phytochrome-mediated responses of phototropism, chlorophyll accumulation and branching are lost. Protoplast transformation with DNA encoding the wild-type protein resulted in a rescue of 0.8% of regenerated protoplasts. In about half of the analyzed lines, formation of CpHO1 concatemers was observed at the CpHO1 locus, whereas in the other half, the mutant CpHO1 gene was replaced by a single DNA copy. This gene repair led to the exchange of single bases, and thus provides the first demonstration of efficient site-directed mutagenesis in a plant nuclear genome. Our studies also revealed an effective mechanism for gene inactivation in Ceratodon. When wild-type protoplasts were transformed with intact or modified CpHO1 genes, approximately 40% of regenerated protoplasts showed the ptr phenotype.
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