ArticleJournal of bacteriology2004
Conserved repeat motifs and glucan binding by glucansucrases of oral streptococci and Leuconostoc mesenteroides.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Diversity of enzymes for exopolysaccharide synthesis in the fructophilic honeybee symbiont Apilactobacillus kunkeei.BMC microbiology · 2026Article
- Functional role and folding properties of the glucan-binding domain of oral bacterial glucansucrase.FEBS letters · 2025Article
- Cold induced expression of a novel levansucrase gene sacB1 enhances exopolysaccharide production and stress resilience in Leuconostoc mesenteroides.Scientific reports · 2025Article
- Unprecedented Diversity of the Glycoside Hydrolase Family 70: A Comprehensive Analysis of Sequence, Structure, and Function.Journal of agricultural and food chemistry · 2024Article
- Polyphenol Utilization Proteins in the Human Gut Microbiome.Applied and environmental microbiology · 2022Article
- Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure-Function Relationship Studies and Engineering.Microorganisms · 2021Review
- The structure of the S-layer of Clostridium difficile.Journal of cell communication and signaling · 2018Review
- An Update on the Evolution of Glucosyltransferase (Frontiers in microbiology · 2018Article
- A parts list for fungal cellulosomes revealed by comparative genomics.Nature microbiology · 2017Article
- Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes.Cellular and molecular life sciences : CMLS · 2016Review
- Carbohydrate-binding specificities of potential probiotic Lactobacillus strains in porcine jejunal (IPEC-J2) cells and porcine mucin.Journal of microbiology (Seoul, Korea) · 2016Article
- Structural Insights into the Carbohydrate Binding Ability of an α-(1→2) Branching Sucrase from Glycoside Hydrolase Family 70.The Journal of biological chemistry · 2016Article
- Cariogenicity of Streptococcus mutans glucan-binding protein deletion mutants.Oral health and dental management · 2013Article
- Functional and structural characterization of α-(1->2) branching sucrase derived from DSR-E glucansucrase.The Journal of biological chemistry · 2012Article
- Inhibition of Streptococcus mutans biofilm formation by Streptococcus salivarius FruA.Applied and environmental microbiology · 2011Article
- Identification and characterization of amylase-binding protein C from Streptococcus mitis NS51.Molecular oral microbiology · 2010Article
- LiaS regulates virulence factor expression in Streptococcus mutans.Infection and immunity · 2008Article
- Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria.Microbiology and molecular biology reviews : MMBR · 2006Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Glucansucrases of oral streptococci and Leuconostoc mesenteroides have a common pattern of structural organization and characteristically contain a domain with a series of tandem amino acid repeats in which certain residues are highly conserved, particularly aromatic amino acids and glycine. In some glucosyltransferases (GTFs) the repeat region has been identified as a glucan binding domain (GBD). Such GBDs are also found in several glucan binding proteins (GBP) of oral streptococci that do not have glucansucrase activity. Alignment of the amino acid sequences of 20 glucansucrases and GBP showed the widespread conservation of the 33-residue A repeat first identified in GtfI of Streptococcus downei. Site-directed mutagenesis of individual highly conserved residues in recombinant GBD of GtfI demonstrated the importance of the first tryptophan and the tyrosine-phenylalanine pair in the binding of dextran, as well as the essential contribution of a basic residue (arginine or lysine). A microplate binding assay was developed to measure the binding affinity of recombinant GBDs. GBD of GtfI was shown to be capable of binding glucans with predominantly alpha-1,3 or alpha-1,6 links, as well as alternating alpha-1,3 and alpha-1,6 links (alternan). Western blot experiments using biotinylated dextran or alternan as probes demonstrated a difference between the binding of streptococcal GTF and GBP and that of Leuconostoc glucansucrases. Experimental data and bioinformatics analysis showed that the A repeat motif is distinct from the 20-residue CW motif, which also has conserved aromatic amino acids and glycine and which occurs in the choline-binding proteins of Streptococcus pneumoniae and other organisms.
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