ArticleJournal of bacteriology2004
Genetic evidence that catabolites of the Entner-Doudoroff pathway signal C source repression of the sigma54 Pu promoter of Pseudomonas putida.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 57 citations in OpenAlex.
- Biosynthesis of fragrance 2-phenylethanol from sugars by Pseudomonas putida.Biotechnology for biofuels and bioproducts · 2024Article
- The acetoin assimilation pathway of Pseudomonas putida KT2440 is regulated by overlapping global regulatory elements that respond to nutritional cues.Environmental microbiology · 2023Article
- From General to Specific: Can Pseudomonas Primary Metabolism Be Exploited for Narrow-Spectrum Antibiotics?Chembiochem : a European journal of chemical biology · 2019Article
- Modeling and analysis of flux distributions in the two branches of the phosphotransferase system in Pseudomonas putida.BMC systems biology · 2012Article
- Article
- Fructose 1-phosphate is the preferred effector of the metabolic regulator Cra of Pseudomonas putida.The Journal of biological chemistry · 2011Article
- Regulation of glucose metabolism in Pseudomonas: the phosphorylative branch and entner-doudoroff enzymes are regulated by a repressor containing a sugar isomerase domain.The Journal of biological chemistry · 2009Article
- Evidence of in vivo cross talk between the nitrogen-related and fructose-related branches of the carbohydrate phosphotransferase system of Pseudomonas putida.Journal of bacteriology · 2008Article
- A set of activators and repressors control peripheral glucose pathways in Pseudomonas putida to yield a common central intermediate.Journal of bacteriology · 2008Article
- Simultaneous catabolite repression between glucose and toluene metabolism in Pseudomonas putida is channeled through different signaling pathways.Journal of bacteriology · 2007Article
- Convergent peripheral pathways catalyze initial glucose catabolism in Pseudomonas putida: genomic and flux analysis.Journal of bacteriology · 2007Article
- The phosphotransferase system formed by PtsP, PtsO, and PtsN proteins controls production of polyhydroxyalkanoates in Pseudomonas putida.Journal of bacteriology · 2007Article
- How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.Microbiology and molecular biology reviews : MMBR · 2006Review
- Role of the ptsN gene product in catabolite repression of the Pseudomonas putida TOL toluene degradation pathway in chemostat cultures.Applied and environmental microbiology · 2006Article
- Integration of signals through Crc and PtsN in catabolite repression of Pseudomonas putida TOL plasmid pWW0.Applied and environmental microbiology · 2005Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glucose and other C sources exert an atypical form of catabolic repression on the sigma54-dependent promoter Pu, which drives transcription of an operon for m-xylene degradation encoded by the TOL plasmid pWW0 in Pseudomonas putida. We have used a genetic approach to identify the catabolite(s) shared by all known repressive C sources that appears to act as the intracellular signal that triggers downregulation of Pu. To this end, we reconstructed from genomic data the pathways for metabolism of repressor (glucose, gluconate) and nonrepressor (fructose) C sources. Since P. putida lacks fructose-6-phosphate kinase, glucose and gluconate appear to be metabolized exclusively by the Entner-Doudoroff (ED) pathway, while fructose can be channeled through the Embden-Meyerhof (EM) route. An insertion in the gene fda (encoding fructose-1,6-bisphosphatase) that forces fructose metabolism to be routed exclusively to the ED pathway makes this sugar inhibitory for Pu. On the contrary, a crc mutation known to stimulate expression of the ED enzymes causes the promoter to be less sensitive to glucose. Interrupting the ED pathway by knocking out eda (encoding 2-dehydro-3-deoxyphosphogluconate aldolase) exacerbates the inhibitory effect of glucose in Pu. These observations pinpoint the key catabolites of the ED route, 6-phosphogluconate and/or 2-dehydro-3-deoxyphosphogluconate, as the intermediates that signal Pu repression. This notion is strengthened by the observation that 2-ketogluconate, which enters the ED pathway by conversion into these compounds, is a strong repressor of the Pu promoter.
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