ArticleNucleic acids research2004
RNA silencing in plants by the expression of siRNA duplexes.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 53 citations in OpenAlex.
- Ultrahigh-activity immune inducer from Endophytic Fungi induces tobacco resistance to virus by SA pathway and RNA silencing.BMC plant biology · 2020Article
- Control of brown patch (Rhizoctonia solani) in tall fescue (Festuca arundinacea Schreb.) by host induced gene silencing.Plant cell reports · 2016Article
- Biotechnological strategies and tools for Plum pox virus resistance: trans-, intra-, cis-genesis, and beyond.Frontiers in plant science · 2015Review
- Assessment of RNAi-induced silencing in banana (Musa spp.).BMC research notes · 2014Article
- RNA-CODE: a noncoding RNA classification tool for short reads in NGS data lacking reference genomes.PloS one · 2013Article
- RNAi mediated silencing of a wall associated kinase, OsiWAK1 in Oryza sativa results in impaired root development and sterility due to anther indehiscence: Wall Associated Kinases from Oryza sativa.Physiology and molecular biology of plants : an international journal of functional plant biology · 2011Article
- Silencing of Plum pox virus 5'UTR/P1 sequence confers resistance to a wide range of PPV strains.Plant cell reports · 2010Article
- Silencing potential of viral derived RNAi constructs in Tomato leaf curl virus-AC4 gene suppression in tomato.Transgenic research · 2010Article
- Hairpin RNAs derived from RNA polymerase II and polymerase III promoter-directed transgenes are processed differently in plants.RNA (New York, N.Y.) · 2008Article
- Artificial microRNA-mediated virus resistance in plants.Journal of virology · 2007Article
- The Quest to Understand the Basis and Mechanisms that Control Expression of Introduced Transgenes in Crop Plants.Plant signaling & behavior · 2006Article
- Novel and mechanical stress-responsive MicroRNAs in Populus trichocarpa that are absent from Arabidopsis.The Plant cell · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In animal cells, stable RNA silencing can be achieved by vector-based small interfering RNA (siRNA) expression system, in which Pol III RNA gene promoters are used to drive the expression of short hairpin RNA, however, this has not been demonstrated in plants. Whether Pol III RNA gene promoter is capable of driving siRNA expression in plants is unknown. Here, we report that RNA silencing was achieved in plants through stable expression of short hairpin RNA, which was driven by Pol III RNA gene promoters. Using glucuronidase (GUS) transformed tobacco as a model system, the results demonstrated that 21 nt RNA duplexes, targeting at different sites of GUS gene, were stably expressed under the control of either human H1 or Arabidopsis 7SL RNA gene promoter, and GUS gene was silenced in 80% of siRNA transgenics. The severity of silencing was correlated with the abundance of siRNA expression but independent of the target sites and uridine residue structures in siRNA hairpin transcripts. Thus, the specific expression of siRNA provides a new system for the study of siRNA silencing pathways and functional genomics in plants. Moreover, the effectiveness of the human H1 promoter in a plant background suggested a conserved mechanism underlying Pol III complex functionality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.