ArticleNucleic acids research2004
In vitro selection of Jun-associated proteins using mRNA display.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 51 citations in OpenAlex.
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- Genetic analysis of genes causing hypertension and stroke in spontaneously hypertensive rats: Gene expression profiles in the kidneys.International journal of molecular medicine · 2015Article
- Structural basis for inhibition of the MDM2:p53 interaction by an optimized MDM2-binding peptide selected with mRNA display.PloS one · 2014Article
- In vitro selection of proteins with desired characteristics using mRNA-display.Methods (San Diego, Calif.) · 2013Article
- Development of Next-Generation Peptide Binders Using In vitro Display Technologies and Their Potential Applications.Frontiers in immunology · 2013Article
- IRView: a database and viewer for protein interacting regions.Bioinformatics (Oxford, England) · 2012Article
- An anilinoquinazoline derivative inhibits tumor growth through interaction with hCAP-G2, a subunit of condensin II.PloS one · 2012Article
- mRNA display-based selections using synthetic peptide and natural protein libraries.Methods in molecular biology (Clifton, N.J.) · 2012Article
- A phthalimide derivative that inhibits centrosomal clustering is effective on multiple myeloma.PloS one · 2012Article
- Selection of proteins with desired properties from natural proteome libraries using mRNA display.Nature protocols · 2011Article
- Advantages of mRNA display selections over other selection techniques for investigation of protein-protein interactions.Expert review of proteomics · 2011Review
- In vitro selection of a peptide inhibitor of human IL-6 using mRNA display.Molecular biotechnology · 2011Article
- Identification of amino acids essential for antibody binding by mRNA-display using a random peptide library: an anti-human tumor protein p53 antibody as a model.Molecular biotechnology · 2009Article
- Use of cDNA tiling arrays for identifying protein interactions selected by in vitro display technologies.PloS one · 2008Article
- Affinity selection of DNA-binding protein complexes using mRNA display.Nucleic acids research · 2006Article
- In vitro evolution of single-chain antibodies using mRNA display.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although yeast two-hybrid assay and biochemical methods combined with mass spectrometry have been successfully employed for the analyses of protein-protein interactions in the field of proteomics, these methods encounter various difficulties arising from the usage of living cells, including inability to analyze toxic proteins and restriction of testable interaction conditions. Totally in vitro display technologies such as ribosome display and mRNA display are expected to circumvent these difficulties. In this study, we applied an mRNA display technique to screening for interactions of a basic leucine zipper domain of Jun protein in a mouse brain cDNA library. By performing iterative affinity selection and sequence analyses, we selected 16 novel Jun-associated protein candidates in addition to four known interactors. By means of real-time PCR and pull-down assay, 10 of the 16 newly discovered candidates were confirmed to be direct interactors with Jun in vitro. Furthermore, interaction of 6 of the 10 proteins with Jun was observed in cultured cells by means of co-immunoprecipitation and observation of subcellular localization. These results demonstrate that this in vitro display technology is effective for the discovery of novel protein-protein interactions and can contribute to the comprehensive mapping of protein-protein interactions.
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