ArticleNucleic acids research2004
Rapid analysis of CpG methylation patterns using RNase T1 cleavage and MALDI-TOF.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Unraveling the RNA modification code with mass spectrometry.Molecular omics · 2020Article
- Within-sample co-methylation patterns in normal tissues.BioData mining · 2019Article
- Down-regulation of the expression of CCAAT/enhancer binding protein α gene in cervical squamous cell carcinoma.BMC cancer · 2014Article
- DNA methylation profiling in the clinic: applications and challenges.Nature reviews. Genetics · 2012Review
- CpG methylation analysis--current status of clinical assays and potential applications in molecular diagnostics: a report of the Association for Molecular Pathology.The Journal of molecular diagnostics : JMD · 2009Article
- Technologies in the Whole-Genome Age: MALDI-TOF-Based Genotyping.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2009Article
- Array-based profiling of reference-independent methylation status (aPRIMES) identifies frequent promoter methylation and consecutive downregulation of ZIC2 in pediatric medulloblastoma.Nucleic acids research · 2007Article
- Genomic profiling of CpG methylation and allelic specificity using quantitative high-throughput mass spectrometry: critical evaluation and improvements.Nucleic acids research · 2007Article
- A matrix of 3,4-diaminobenzophenone for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Nucleic acids research · 2006Article
- Matrix-assisted laser desorption/ionisation, time-of-flight mass spectrometry in genomics research.PLoS genetics · 2006Review
- Rapid quantification of DNA methylation through dNMP analysis following bisulfite-PCR.Nucleic acids research · 2006Article
- Novel method for high throughput DNA methylation marker evaluation using PNA-probe library hybridization and MALDI-TOF detection.Nucleic acids research · 2006Article
- Precision and performance characteristics of bisulfite conversion and real-time PCR (MethyLight) for quantitative DNA methylation analysis.The Journal of molecular diagnostics : JMD · 2006Article
- Techniques used in studies of epigenome dysregulation due to aberrant DNA methylation: an emphasis on fetal-based adult diseases.Reproductive toxicology (Elmsford, N.Y.)Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we introduce a method for the fast and accurate analysis of DNA methylation based on bisulfite-treated DNA. The target region is PCR amplified using a T7 RNA polymerase promoter-tagged primer. A subsequent in vitro transcription leads to a transcript which contains guanosine residues only at sites that contained methylated cytosines before bisulfite treatment. In a single tube reaction using guanosine-specific cleavage by RNase T1, a specific pattern of RNA fragments is formed. This pattern directly represents the methylation state of the sample DNA and is analyzed using matrix-assisted laser desorption ionization time-of-flight technology. This method was successfully applied to the analysis of artificially methylated and unmethylated DNA, mixtures thereof and colon DNA samples. The applicability for the analysis of both PCR products and cloned PCR products is demonstrated. The observed methylation patterns were confirmed by bisulfite sequencing.
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