ArticleNucleic acids research2004
NFI and Oct-1 bend the Ad5 origin in the same direction leading to optimal DNA replication.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Viral and cellular interactions during adenovirus DNA replication.FEBS letters · 2019Review
- Article
- Studying protein-DNA interactions using atomic force microscopy.Seminars in cell & developmental biology · 2018Review
- Cis-acting DNA sequence at a replication origin promotes repeat expansion to fragile X full mutation.The Journal of cell biology · 2014Article
- Overexpression of Ad5 precursor terminal protein accelerates recombinant adenovirus packaging and amplification in HEK-293 packaging cells.Gene therapy · 2014Article
- Adenovirus DNA replication.Cold Spring Harbor perspectives in biology · 2013Review
- Stimulation of BK virus DNA replication by NFI family transcription factors.Journal of virology · 2012Article
- Phosphoacceptor site S173 in the regulatory domain of Epstein-Barr Virus ZEBRA protein is required for lytic DNA replication but not for activation of viral early genes.Journal of virology · 2007Article
- Essential elements of a licensed, mammalian plasmid origin of DNA synthesis.Molecular and cellular biology · 2006Article
- Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen induces a strong bend on binding to terminal repeat DNA.Journal of virology · 2005Article
- The POU transcription factor Oct-1 represses virus-induced interferon A gene expression.Molecular and cellular biology · 2005Article
- Analysis of scanning force microscopy images of protein-induced DNA bending using simulations.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two cellular transcription factors, nuclear factor I (NFI) and octamer binding protein (Oct-1), bind simultaneously to their recognition sequences in the Ad5 origin of replication thereby enhancing initiation. Using scanning force microscopy we have previously shown that NFI induces a 60 degrees bend in the origin DNA. Here we demonstrate that Oct-1 induces a 42 degrees bend in the origin DNA. Simultaneous binding of NFI and Oct-1 induces an 82 degrees collective bend suggesting that both bends are oriented towards each other. In functional replication assays we further demonstrate that this extensive DNA bending leads to a synergistic enhancement of DNA replication. We propose that collective DNA bending induced by NFI and Oct-1 facilitates the optimal assembly of the preinitiation complex and plays an important role in the stimulatory mechanism of NFI and Oct-1 in replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.