ArticleNucleic acids research2004
Complete set of orthogonal 21st aminoacyl-tRNA synthetase-amber, ochre and opal suppressor tRNA pairs: concomitant suppression of three different termination codons in an mRNA in mammalian cells.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- An engineered glutamic acid tRNA for efficient suppression of pathogenic nonsense mutations.Nucleic acids research · 2025Article
- Engineering a controllable and reversible switch for CAR-based cellular immunotherapies via a genetic code expansion system.Journal of hematology & oncology · 2024Article
- Cell Surface Engineering Tools for Programming Living Assemblies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Article
- Using genetically incorporated unnatural amino acids to control protein functions in mammalian cells.Essays in biochemistry · 2019Review
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- Aminoacyl-tRNA Synthetases in the Bacterial World.EcoSal Plus · 2016Review
- Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis.Journal of the American Chemical Society · 2016Article
- Nonsense suppression in archaea.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A general approach to site-specific antibody drug conjugates.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Diversity of human tRNA genes from the 1000-genomes project.RNA biology · 2013Article
- Near-cognate suppression of amber, opal and quadruplet codons competes with aminoacyl-tRNAPyl for genetic code expansion.FEBS letters · 2012Article
- Genetically encoded libraries of nonstandard peptides.Journal of nucleic acids · 2012Article
- Regulatory RNAs derived from transfer RNA?RNA (New York, N.Y.) · 2010Review
- Article
- In vitro incorporation of nonnatural amino acids into protein using tRNA(Cys)-derived opal, ochre, and amber suppressor tRNAs.RNA (New York, N.Y.) · 2010Article
- Functional analysis of human tRNA isodecoders.Journal of molecular biology · 2010Article
- Splicing of mRNA mediated by tRNA sequences in mouse cells.RNA (New York, N.Y.) · 2009Article
- Quality control despite mistranslation caused by an ambiguous genetic code.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The many applications of acid urea polyacrylamide gel electrophoresis to studies of tRNAs and aminoacyl-tRNA synthetases.Methods (San Diego, Calif.) · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We describe the generation of a complete set of orthogonal 21st synthetase-amber, ochre and opal suppressor tRNA pairs including the first report of a 21st synthetase-ochre suppressor tRNA pair. We show that amber, ochre and opal suppressor tRNAs, derived from Escherichia coli glutamine tRNA, suppress UAG, UAA and UGA termination codons, respectively, in a reporter mRNA in mammalian cells. Activity of each suppressor tRNA is dependent upon the expression of E.coli glutaminyl-tRNA synthetase, indicating that none of the suppressor tRNAs are aminoacylated by any of the twenty aminoacyl-tRNA synthetases in the mammalian cytoplasm. Amber, ochre and opal suppressor tRNAs with a wide range of activities in suppression (increases of up to 36, 156 and 200-fold, respectively) have been generated by introducing further mutations into the suppressor tRNA genes. The most active suppressor tRNAs have been used in combination to concomitantly suppress two or three termination codons in an mRNA. We discuss the potential use of these 21st synthetase-suppressor tRNA pairs for the site-specific incorporation of two or, possibly, even three different unnatural amino acids into proteins and for the regulated suppression of amber, ochre and opal termination codons in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.