ArticleApplied and environmental microbiology2004
Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 114 citations in OpenAlex.
- Review of the Proteomics and Metabolic Properties ofMicroorganisms · 2024Review
- GC/MS-basedMicrobial cell factories · 2022Article
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- Complete and Draft Genome Sequences of Amino Acid-Producing Corynebacterium glutamicum Strains ATCC 21799 and ATCC 31831 and Their Genomic Islands.Microbiology resource announcements · 2020Article
- Construction and analysis of an artificial consortium based on the fast-growing cyanobacteriumBiotechnology for biofuels · 2020Article
- Beta-elemene inhibits breast cancer metastasis through blocking pyruvate kinase M2 dimerization and nuclear translocation.Journal of cellular and molecular medicine · 2019Article
- The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis.The Journal of biological chemistry · 2014Article
- Improvement of cell growth and L-lysine production by genetically modified Corynebacterium glutamicum during growth on molasses.Journal of industrial microbiology & biotechnology · 2013Article
- Lysine overproducing Corynebacterium glutamicum is characterized by a robust linear combination of two optimal phenotypic states.Systems and synthetic biology · 2013Article
- Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine.Microbial cell factories · 2012Article
- Comparison between elementary flux modes analysis and 13C-metabolic fluxes measured in bacterial and plant cells.BMC systems biology · 2011Article
- Article
- (13)C-based metabolic flux analysis.Nature protocols · 2009Article
- Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum.Microbial cell factories · 2008Article
- Fluxome analysis using GC-MS.Microbial cell factories · 2007Article
- Transcriptional and metabolic responses of Bacillus subtilis to the availability of organic acids: transcription regulation is important but not sufficient to account for metabolic adaptation.Applied and environmental microbiology · 2007Article
- Metabolic networks in motion: 13C-based flux analysis.Molecular systems biology · 2006Review
- Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources.Applied and environmental microbiology · 2005Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic fluxes in the central metabolism were determined for lysine-producing Corynebacterium glutamicum ATCC 21526 with sucrose as a carbon source, providing an insight into molasses-based industrial production processes with this organism. For this purpose, 13C metabolic flux analysis with parallel studies on [1-(13C)Fru]sucrose, [1-(13C)Glc]sucrose, and [13C6Fru]sucrose was carried out. C. glutamicum directed 27.4% of sucrose toward extracellular lysine. The strain exhibited a relatively high flux of 55.7% (normalized to an uptake flux of hexose units of 100%) through the pentose phosphate pathway (PPP). The glucose monomer of sucrose was completely channeled into the PPP. After transient efflux, the fructose residue was mainly taken up by the fructose-specific phosphotransferase system (PTS) and entered glycolysis at the level of fructose-1,6-bisphosphate. Glucose-6-phosphate isomerase operated in the gluconeogenetic direction from fructose-6-phosphate to glucose-6-phosphate and supplied additional carbon (7.2%) from the fructose part of the substrate toward the PPP. This involved supply of fructose-6-phosphate from the fructose part of sucrose either by PTS(Man) or by fructose-1,6-bisphosphatase. C. glutamicum further exhibited a high tricarboxylic acid (TCA) cycle flux of 78.2%. Isocitrate dehydrogenase therefore significantly contributed to the total NADPH supply of 190%. The demands for lysine (110%) and anabolism (32%) were lower than the supply, resulting in an apparent NADPH excess. The high TCA cycle flux and the significant secretion of dihydroxyacetone and glycerol display interesting targets to be approached by genetic engineers for optimization of the strain investigated.
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