ArticleMolecular biology of the cell2005
Cdc42 and RhoB activation are required for mannose receptor-mediated phagocytosis by human alveolar macrophages.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 82 citations in OpenAlex.
- Refined phenotyping of vaccine responses reveals transcriptomic determinants of neutralizing antibody heterogeneity.NPJ vaccines · 2026Article
- Phagocytosis at a glance.Journal of cell science · 2025Review
- Proteomic characterisation of perhexiline treatment on THP-1 M1 macrophage differentiation.Frontiers in immunology · 2023Article
- The mannose receptor ligands and the macrophage glycome.Current opinion in structural biology · 2022Review
- The CD33 short isoform is a gain-of-function variant that enhances AβMolecular neurodegeneration · 2021Article
- Functional and Therapeutic Relevance of Rho GTPases in Innate Immune Cell Migration and Function during Inflammation: AnMediators of inflammation · 2021Review
- Intestinal Metabolites Influence Macrophage Phagocytosis and Clearance of Bacterial Infection.Frontiers in cellular and infection microbiology · 2021Article
- miR-223-3p Inhibits Antigen Endocytosis and Presentation and Promotes the Tolerogenic Potential of Dendritic Cells through Targeting Mannose Receptor Signaling and Rhob.Journal of immunology research · 2020Article
- Pneumolysin and the bacterial capsule of Streptococcus pneumoniae cooperatively inhibit taxis and motility of microglia.Journal of neuroinflammation · 2019Article
- Review
- The RhoB small GTPase in physiology and disease.Small GTPases · 2018Review
- Epigenetic alterations are associated with monocyte immune dysfunctions in HIV-1 infection.Scientific reports · 2018Article
- The Interaction of Human Pathogenic Fungi With C-Type Lectin Receptors.Frontiers in immunology · 2018Review
- M. tuberculosis-Initiated Human Mannose Receptor Signaling Regulates Macrophage Recognition and Vesicle Trafficking by FcRγ-Chain, Grb2, and SHP-1.Cell reports · 2017Article
- MicroRNA-21 Limits Uptake ofFrontiers in cellular and infection microbiology · 2017Article
- The Mannose Receptor Is Involved in the Phagocytosis of Mycobacteria-Induced Apoptotic Cells.Journal of immunology research · 2016Article
- Single-cell analysis reveals differential regulation of the alveolar macrophage actin cytoskeleton by surfactant proteins A1 and A2: implications of sex and aging.Biology of sex differences · 2016Article
- Humoral immune responses to Streptococcus pneumoniae in the setting of HIV-1 infection.Vaccine · 2015Review
- Macrophage-Targeting Gene Delivery Using a Micelle Composed of Mannose-Modified Lipid with Triazole Ring and Dioleoyl Trimethylammonium Propane.BioMed research international · 2015Article
- EGF receptor-dependent mechanism may be involved in the Tamm-Horsfall glycoprotein-enhanced PMN phagocytosis via activating Rho family and MAPK signaling pathway.Molecules (Basel, Switzerland) · 2014Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Human alveolar macrophages (AMs) phagocytose Pneumocystis (Pc) organisms predominantly through mannose receptors, although the molecular mechanism mediating this opsonin-independent process is not known. In this study, using AMs from healthy individuals, Pc phagocytosis was associated with focal F-actin polymerization and Cdc42, Rac1, and Rho activation in a time-dependent manner. Phagocytosis was primarily dependent on Cdc42 and RhoB activation (as determined by AM transfection with Cdc42 and RhoB dominant-negative alleles) and mediated predominantly through mannose receptors (as determined by siRNA gene silencing of AM mannose receptors). Pc also promoted PAK-1 phosphorylation, which was also dependent on RhoGTPase activation. HIV infection of AMs (as a model for reduced mannose receptor expression and function) was associated with impaired F-actin polymerization, reduced Cdc42 and Rho activation, and markedly reduced PAK-1 phosphorylation in response to Pc organisms. In healthy AMs, Pc phagocytosis was partially dependent on PAK activation, but dependent on the Rho effector molecule ROCK. These data provide a molecular mechanism for AM mannose receptor-mediated phagocytosis of unopsonized Pc organisms that appears distinct from opsonin-dependent phagocytic receptors. Reduced AM mannose receptor-mediated Cdc42 and Rho activation in the context of HIV infection may represent a mechanism that contributes to the pathogenesis of opportunistic pneumonia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.