ArticleGenome research2004
Novel transcription regulatory elements in Caenorhabditis elegans muscle genes.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
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- Identification of Predictive Cis-Regulatory Elements Using a Discriminative Objective Function and a Dynamic Search Space.PloS one · 2015Article
- Transcription factor redundancy and tissue-specific regulation: evidence from functional and physical network connectivity.Genome research · 2012Article
- Conserved Motifs and Prediction of Regulatory Modules in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2012Article
- A method for rapid and simultaneous mapping of genetic loci and introgression sizes in nematode species.PloS one · 2012Article
- Modeling the quantitative specificity of DNA-binding proteins from example binding sites.PloS one · 2009Article
- Caenorhabditis elegans cisRED: a catalogue of conserved genomic elements.Nucleic acids research · 2009Article
- Exuberant expression of chemokine genes by adult human articular chondrocytes in response to IL-1beta.Osteoarthritis and cartilage · 2008Article
- Poly-G/poly-C tracts in the genomes of Caenorhabditis.BMC genomics · 2007Article
- Article
- oPOSSUM: integrated tools for analysis of regulatory motif over-representation.Nucleic acids research · 2007Article
- Identification of muscle-specific regulatory modules in Caenorhabditis elegans.Genome research · 2007Article
- A systematic model to predict transcriptional regulatory mechanisms based on overrepresentation of transcription factor binding profiles.Genome research · 2006Article
- Computational identification of transcriptional regulatory elements in DNA sequence.Nucleic acids research · 2006Review
- De novo discovery of a tissue-specific gene regulatory module in a chordate.Genome research · 2005Article
- Discovery, validation, and genetic dissection of transcription factor binding sites by comparative and functional genomics.Genome research · 2005Article
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4 authors at 2 institutions in 1 country.
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Abstract
We report the identification of three new transcription regulatory elements that are associated with muscle gene expression in the nematode Caenorhabditis elegans. Starting from a subset of well-characterized nematode muscle genes, we identified conserved DNA motifs in the promoter regions using computational DNA pattern-recognition algorithms. These were considered to be putative muscle transcription regulatory motifs. Using the green-fluorescent protein (GFP) as a reporter, experiments were done to determine the biological activity of these motifs in driving muscle gene expression. Prediction accuracy of muscle expression based on the presence of these three motifs was encouraging; nine of 10 previously uncharacterized genes that were predicted to have muscle expression were shown to be expressed either specifically or selectively in the muscle tissues, whereas only one of the nine that scored low for these motifs expressed in muscle. Knockouts of putative regulatory elements in the promoter of the mlc-2 and unc-89 genes show that they significantly contribute to muscle expression and act in a synergistic manner. We find that these DNA motifs are also present in the muscle promoters of C. briggsae, indicating that they are functionally conserved in the nematodes.
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