ArticleMolecular and cellular biology2004
Evidence for histone eviction in trans upon induction of the yeast PHO5 promoter.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 95 citations in OpenAlex.
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- Genome organization across scales: mechanistic insights from in vitro reconstitution studies.Biochemical Society transactions · 2024Review
- SWI/SNF Chromatin Remodelers: Structural, Functional and Mechanistic Implications.Cell biochemistry and biophysics · 2023Review
- The role of 3D genome organization in disease: From compartments to single nucleotides.Seminars in cell & developmental biology · 2019Review
- NicE-seq: high resolution open chromatin profiling.Genome biology · 2017Article
- Nucleosomal signatures impose nucleosome positioning in coding and noncoding sequences in the genome.Genome research · 2016Article
- Re-establishment of nucleosome occupancy during double-strand break repair in budding yeast.DNA repair · 2016Article
- ASF1 and the SWI/SNF complex interact functionally during nucleosome displacement, while FACT is required for nucleosome reassembly at yeast heat shock gene promoters during sustained stress.Cell stress & chaperones · 2015Article
- Replication-guided nucleosome packing and nucleosome breathing expedite the formation of dense arrays.Nucleic acids research · 2014Article
- The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening.Nucleic acids research · 2014Article
- Chromatin accessibility: a window into the genome.Epigenetics & chromatin · 2014Review
- The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin.Nucleic acids research · 2014Review
- Recombination-induced tag exchange (RITE) cassette series to monitor protein dynamics in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2013Article
- Chromatin and the genome integrity network.Nature reviews. Genetics · 2013Review
- Rad26p regulates the occupancy of histone H2A-H2B dimer at the active genes in vivo.Nucleic acids research · 2012Article
- Chromatin and transcription in yeast.Genetics · 2012Review
- DNA Topoisomerases maintain promoters in a state competent for transcriptional activation in Saccharomyces cerevisiae.PLoS genetics · 2012Article
- GAL1-SceI directed site-specific genomic (gsSSG) mutagenesis: a method for precisely targeting point mutations in S. cerevisiae.BMC biotechnology · 2011Article
- Diversity of operation in ATP-dependent chromatin remodelers.Biochimica et biophysica acta · 2011Review
- Simultaneous single-molecule mapping of protein-DNA interactions and DNA methylation by MAPit.Current protocols in molecular biology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast PHO5 promoter is a model system for the role of chromatin in eukaryotic gene regulation. Four positioned nucleosomes in the repressed state give way to an extended DNase I hypersensitive site upon induction. Recently this hypersensitive site was shown to be devoid of histone DNA contacts. This raises the mechanistic question of how histones are removed from the promoter. A displacement in trans or movement in cis, the latter according to the well established nucleosome sliding mechanism, are the major alternatives. In this study, we embedded the PHO5 promoter into the context of a small plasmid which severely restricts the space for nucleosome sliding along the DNA in cis. Such a construct would either preclude the chromatin transition upon induction altogether, were it to occur in cis, or gross changes in chromatin around the plasmid would be the consequence. We observed neither. Instead, promoter opening on the plasmid was indistinguishable from opening at the native chromosomal locus. This makes a sliding mechanism for the chromatin transition at the PHO5 promoter highly unlikely and points to histone eviction in trans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.