ArticleMolecular and cellular biology2004
Counting of Rif1p and Rif2p on Saccharomyces cerevisiae telomeres regulates telomere length.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
79 citing papers in PubMed.
- Rap1-mediated steric hindrance protects telomeres from MRX sensing.Nature structural & molecular biology · 2026Article
- Natural diversity of telomere length distributions across 100Genome research · 2026Article
- Article
- Human telomere length is chromosome end-specific and conserved across individuals.Science (New York, N.Y.) · 2024Article
- Rif2 interaction with Rad50 counteracts Tel1 functions in checkpoint signalling and DNA tethering by releasing Tel1 from MRX binding.Nucleic acids research · 2024Article
- Rif1 Regulates Self-Renewal and Impedes Mesendodermal Differentiation of Mouse Embryonic Stem Cells.Stem cell reviews and reports · 2023Article
- Post-Transcriptional and Post-Translational Modifications in Telomerase Biogenesis and Recruitment to Telomeres.International journal of molecular sciences · 2023Review
- Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans.Microbiology spectrum · 2022Article
- Rif1-Dependent Control of Replication Timing.Genes · 2022Review
- Telomere length regulation by Rif1 protein fromeLife · 2022Article
- Article
- Mechanism of MRX inhibition by Rif2 at telomeres.Nature communications · 2021Article
- The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1.Nature communications · 2021Article
- Rif1 regulates telomere length through conserved HEAT repeats.Nucleic acids research · 2021Article
- A unified alternative telomere-lengthening pathway in yeast survivor cells.Molecular cell · 2021Article
- Sae2 and Rif2 regulate MRX endonuclease activity at DNA double-strand breaks in opposite manners.Cell reports · 2021Article
- The telomere-binding protein Rif2 and ATP-bound Rad50 have opposing roles in the activation of yeast Tel1The Journal of biological chemistry · 2019Article
- Processing of DNA Double-Strand Breaks by the MRX Complex in a Chromatin Context.Frontiers in molecular biosciences · 2019Review
- Review
- The evolutionarily conserved factor Sus1/ENY2 plays a role in telomere length maintenance.Current genetics · 2018Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Telomere length is negatively regulated by proteins of the telomeric DNA-protein complex. Rap1p in Saccharomyces cerevisiae binds the telomeric TG(1-3) repeat DNA, and the Rap1p C terminus interacts with Rif1p and Rif2p. We investigated how these three proteins negatively regulate telomere length. We show that direct tethering of each Rif protein to a telomere shortens that telomere proportionally to the number of tethered molecules, similar to previously reported counting of Rap1p. Surprisingly, Rif proteins could also regulate telomere length even when the Rap1p C terminus was absent, and tethered Rap1p counting was completely dependent on the Rif proteins. Thus, Rap1p counting is in fact Rif protein counting. In genetic settings that cause telomeres to be abnormally long, tethering even a single Rif2p molecule was sufficient for maximal effectiveness in preventing the telomere overelongation. We show that a heterologous protein oligomerization domain, the mammalian PDZ domain, when fused to Rap1p can confer telomere length control. We propose that a nucleation and spreading mechanism is involved in forming the higher-order telomere structure that regulates telomere length.
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Registered trials
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