ArticleMolecular and cellular biology2004
C-terminal anchoring of mid1p to membranes stabilizes cytokinetic ring position in early mitosis in fission yeast.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- PP2A-B56 regulates Mid1 protein levels for proper cytokinesis in fission yeast.Molecular biology of the cell · 2025Article
- Anillin-related Mid1 as an adaptive and multimodal contractile ring anchoring protein: A simulation study.Structure (London, England : 1993) · 2024Article
- Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion.Cell reports · 2023Article
- Molecular organization of cytokinesis node predicts the constriction rate of the contractile ring.The Journal of cell biology · 2021Article
- A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast.eLife · 2020Article
- Fission yeast Pak1 phosphorylates anillin-like Mid1 for spatial control of cytokinesis.The Journal of cell biology · 2020Article
- The Functionally Important N-Terminal Half of Fission Yeast Mid1p Anillin Is Intrinsically Disordered and Undergoes Phase Separation.Biochemistry · 2019Article
- Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019Review
- NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement.Current biology : CB · 2019Article
- Rho Family GTPases in Fission Yeast Cytokinesis.Communicative & integrative biology · 2019Review
- Analysis of interphase node proteins in fission yeast by quantitative and superresolution fluorescence microscopy.Molecular biology of the cell · 2017Article
- Cytokinesis in Metazoa and Fungi.Cold Spring Harbor perspectives in biology · 2017Review
- Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis.The Journal of cell biology · 2017Article
- Controlling cell size through sizer mechanisms.Current opinion in systems biology · 2017Review
- Mechanoregulated inhibition of formin facilitates contractile actomyosin ring assembly.Nature communications · 2017Article
- Nanoscale architecture of theeLife · 2017Article
- Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A Plasma Membrane Association Module in Yeast Amino Acid Transporters.The Journal of biological chemistry · 2016Article
- Comparative biology of cell division in the fission yeast clade.Current opinion in microbiology · 2015Review
- Mechanistic insights into the anchorage of the contractile ring by anillin and Mid1.Developmental cell · 2015Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
mid1p is a key factor for the central positioning of the cytokinetic ring in Schizosaccharomyces pombe. In interphase and early mitosis, mid1p forms a medial cortical band overlying the nucleus, which may represent a landmark for cytokinetic ring assembly. It compacts before anaphase into a tight ring with other cytokinetic ring components. We show here that mid1p binds to the medial cortex by at least two independent means. First, mid1p C-terminus association with the cortex requires a putative amphipathic helix adjacent to mid1p nuclear localization sequence (NLS), which is predicted to insert directly into the lipid bilayer. This association is stabilized by the polybasic NLS. mid1p mutated within the helix and the NLS forms abnormal filaments in early mitosis that are not properly anchored to the medial cortex. Misplaced rings assemble in late mitosis, indicating that mid1p C-terminus binding to membranes stabilizes cytokinetic ring position. Second, the N terminus of mid1p has the ability to associate faintly with the medial cortex and is sufficient to form tight rings. In addition, we show that mid1p oligomerizes. We propose that membrane-bound oligomers of mid1p assemble recruitment "platforms" for cytokinetic ring components at the medial cortex and stabilize the ring position during its compaction.
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