ArticleProceedings of the National Academy of Sciences of the United States of America2004
Single-cell FRET imaging of phosphatase activity in the Escherichia coli chemotaxis system.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 106 citations in OpenAlex.
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- The architecture of redox microdomains: Cascading gradients and peroxiredoxins' redox-oligomeric coupling integrate redox signaling and antioxidant protection.Redox biology · 2024Article
- The Chemoreceptor Sensory Adaptation System Produces Coordinated Reversals of the Flagellar Motors on an Escherichia coli Cell.Journal of bacteriology · 2022Article
- Suppression of cell-cell variation by cooperative interaction of phosphatase and response regulator.Biophysical journal · 2022Article
- Modular and Single-Cell Sensors of Bacterial Ser/Thr Kinase Activity.ACS synthetic biology · 2021Article
- Bacterial cell-body rotation driven by a single flagellar motor and by a bundle.Biophysical journal · 2021Article
- Fluctuations in Intracellular CheY-P Concentration Coordinate Reversals of Flagellar Motors inBiomolecules · 2020Article
- Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ inFrontiers in microbiology · 2020Article
- New Twists and Turns in Bacterial Locomotion and Signal Transduction.Journal of bacteriology · 2019Review
- Probing chemotaxis activity in Escherichia coli using fluorescent protein fusions.Scientific reports · 2019Article
- Rotating Bacteria on Solid Surfaces without Tethering.Biophysical journal · 2018Article
- Behavioral Variability and Phenotypic Diversity in Bacterial Chemotaxis.Annual review of biophysics · 2018Review
- Phenotypic diversity and temporal variability in a bacterial signaling network revealed by single-cell FRET.eLife · 2017Article
- Recent advances and future prospects in bacterial and archaeal locomotion and signal transduction.Journal of bacteriology · 2017Article
- Bacterial protein networks: properties and functions.Nature reviews. Microbiology · 2015Review
- Fundamental constraints on the abundances of chemotaxis proteins.Biophysical journal · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Two-component signaling systems, in which a receptor-coupled kinase is used to control the phosphorylation level of a response regulator, are commonly used in bacteria to sense their environment. In the chemotaxis system of Escherichia coli, the receptors, and thus the kinase, are clustered on the inner cell membrane. The phosphatase of this system also is recruited to receptor clusters, but the reason for this association is not clear. By using FRET imaging of single cells, we show that in vivo the phosphatase activity is substantially larger at the cluster, indicating that the signaling source (the kinase) and the signaling sink (the phosphatase) tend to be located at the same place in the cell. When this association is disrupted, a gradient in the concentration of the phosphorylated response regulator appears, and the chemotactic response is degraded. Such colocalization is inevitable in systems in which the activity of the kinase and the phosphatase are produced by the same enzyme. Evidently, this design enables a more rapid and spatially uniform response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.